|
| Status |
Public on Dec 31, 2008 |
| Title |
ET_cells_4h_Dioxane_2 |
| Sample type |
RNA |
| |
|
| Source name |
Fetal murine cortical thymic epithelial cell line
|
| Organism |
Mus musculus |
| Characteristics |
Fetal murine cortical thymic epithelial cell line
Treatment: 0.05 %V 1.4_Dioxane
Time: 4h
Replicate: 2
|
| Treatment protocol |
80-90% confluent ET cells were washed with PBS and treated with 0.05% V 1.4-Dioxane in DMEM (10%FCS, Glucose high). After 4h cells were washed with PBS and RNA isolated with TRIzol.
|
| Growth protocol |
The ET, fetal murine cortical thymic epithelial cell line, was cultivated to 80-90 % confluence on 24-well plates. Cells were cultivated in DMEM, glucose high, containing L-glutamine (PAA), was supplemented with 10% FCS and 1%pen/strep.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from 80-90% confluent ET cells using TRIzol and after insuring integrity processed after the Affymetrix standard protocol
|
| Label |
PE
|
| Label protocol |
Affymetrix standard protocol
|
| |
|
| Hybridization protocol |
Affymetrix standard protocol
|
| Scan protocol |
Affymetrix standard protocol
|
| Description |
ET-cells were treated with Dioxane for 4h
|
| Data processing |
Data was processed using RMA (affy-package, R 2.4)
|
| |
|
| Submission date |
Jan 08, 2008 |
| Last update date |
Mar 18, 2009 |
| Contact name |
Markus Frericks |
| E-mail(s) |
fremar5@web.de
|
| Phone |
+49(0)211 3389253
|
| Fax |
+49(0)211 3190910
|
| Organization name |
Institute for Environmental Research
|
| Department |
Immunology
|
| Street address |
Auf'm Hennekamp 50
|
| City |
Duesseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE10168 |
AhR activation by TCDD in the ET, cortical epithelial cell line |
|
