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| Status |
Public on Jan 01, 2009 |
| Title |
Collateral Flow Evokes Distinct Endothelial Gene Expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Biomechanical forces influence vascular function, but the molecular mechanisms regulating many of these mechano-activated cellular events remain largely uncharacterized. In particular, the vascular endothelium can sense alterations in hemodynamic shear stress which, in the context of adaptive remodeling or arteriogenesis, has been shown to lead to the generation of endothelial-derived signals critical for controlling blood vessel structure and function. Here, we sought to define the arteriogenic responses evoked in endothelial cells exposed to flow using transciptional profiling. Analysis of these transcriptional programs identified several genes previously shown to be important for endothelial-smooth muscle interactions and vascular remodeling, including the transcription factor kruppel-like factor 2 (KLF2).
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| Overall design |
A lumped parameter model of the human coronary collateral circulation was used to simulate previously uncharacterized normal (NCC) and adaptive remodeling (ACC) coronary collateral shear stress waveforms. These waveforms were then applied to cultured human endothelial cells (EC) and the resulting differences in EC gene expression were assessed by genome-wide transcriptional profiling to identify genes distinctly regulated by collateral flow.
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| |
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| Contributor(s) |
Mack PJ, Zhang Y, Kamm RD, Garcia-Cardena G |
| Citation(s) |
19047056 |
| |
| Submission date |
May 28, 2008 |
| Last update date |
Jan 27, 2016 |
| Contact name |
Peter J Mack |
| E-mail(s) |
peter_mack@hms.harvard.edu
|
| Phone |
617-525-4339
|
| Fax |
617-525-4329
|
| Organization name |
Harvard Medical School
|
| Department |
Pathology
|
| Lab |
Center for Excullence in Vascular Biology
|
| Street address |
77 Avenue Louis Pasteur, NRB-752
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL2986 |
ABI Human Genome Survey Microarray Version 2 |
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| Samples (6)
|
| GSM291580 |
Normal collateral EC gene expression N1 |
| GSM291581 |
Adaptive remodeling collateral EC gene expression N1 |
| GSM291582 |
Normal collateral EC gene expression N2 |
| GSM291583 |
Adaptive remodeling collateral EC gene expression N2 |
| GSM291584 |
Normal collateral EC gene expression N3 |
| GSM291585 |
Adaptive remodeling collateral EC gene expression N3 |
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| Relations |
| BioProject |
PRJNA106215 |
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