|
| Status |
Public on Jan 01, 2009 |
| Title |
Adaptive remodeling collateral EC gene expression N3 |
| Sample type |
RNA |
| |
|
| Source name |
human umbilical vein endothelial cells, adaptive remodeling collateral EC gene expression N3
|
| Organism |
Homo sapiens |
| Characteristics |
primary human umbilical vein endothelial cells from pooled sources
|
| Biomaterial provider |
BWH Vascular Research Division
|
| Treatment protocol |
Approximately 5e6 endothelial cells were subjected to simulated adaptive remodeling coronary collateral waveforms for 24 hr.
|
| Growth protocol |
Medium-199, 2% FBS, 2 mM L-Glutamine, 100 units/ml penicillin plus 100 m g/ml streptomycin, and 2.8 % dextran (Invitrogen)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
40 µg of total RNA, extracted using Trizol (Invitrogen) and RNeasy Purication (Quiagen) according to the manufacturer’s instructions
|
| Label |
Digoxigenin
|
| Label protocol |
Digoxigenin labeling into the cDNA products (ABI protocol)
|
| |
|
| Hybridization protocol |
ABI protocol
|
| Scan protocol |
ABI protocol
|
| Description |
Transcriptional profiling was performed with endothelial cells subjected to simulated coronary collateral waveforms using an
|
| Data processing |
ABI 1700 chemiluminescent microarray system and analyzer. Post-processing of data with Lowess regression and Z-pool variances was performed with custom statistical software.
|
| |
|
| Submission date |
May 28, 2008 |
| Last update date |
May 29, 2008 |
| Contact name |
Peter J Mack |
| E-mail(s) |
peter_mack@hms.harvard.edu
|
| Phone |
617-525-4339
|
| Fax |
617-525-4329
|
| Organization name |
Harvard Medical School
|
| Department |
Pathology
|
| Lab |
Center for Excullence in Vascular Biology
|
| Street address |
77 Avenue Louis Pasteur, NRB-752
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL2986 |
| Series (1) |
| GSE11583 |
Collateral Flow Evokes Distinct Endothelial Gene Expression |
|
