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| Status |
Public on Jun 30, 2019 |
| Title |
Leo1 is essential for dynamic regulation of heterochromatin and gene expression during cellular quiescence [RNA-Seq] |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Cellular quiescence is a reversible differentiation state when cells are changing the gene expression programme to reduce metabolic functions and adapt to a new cellular environment. The epigenetic changes that accompany these alterations are not so well understood. Here we investigate the role of Leo1, a subunit of the conserved Paf1 (RNA polymerase-associated factor 1) complex, in the quiescence process using fission yeast as a model organism. Fission yeast cells enter the G0 phase of the cell cycle when exposed to nitrogen starvation and the heterochromatin regions become very dynamic. The reduction of heterochromatin in early G0 correlates with the reduced activity of target of rapamycin, TORC2, signalling. Cells lacking the Leo1 show reduced survival in G0. In these cells heterochromatin regions including subtelomeres are stabilized and many genes, including membrane transport genes, fail to be expressed. Our results suggest that Leo1 is essential for dynamic regulation of heterochromatin and gene expression during cellular quiescence.
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| Overall design |
RNA profiles in wt/leo1D during quiescence; total 20 samples; biological duplicates
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| Contributor(s) |
Oya E, Dubief MD, Cohen A, Schurra C, Nakayama J, Weisman R, Arcangioli B, Ekwall K |
| Citation(s) |
31315658 |
| |
| Submission date |
Jul 05, 2018 |
| Last update date |
Sep 29, 2019 |
| Contact name |
Eriko Oya |
| Organization name |
Karolinska Institutet
|
| Street address |
Blickagången 16
|
| City |
Huddinge |
| ZIP/Postal code |
141 83 |
| Country |
Sweden |
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| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA479830 |
| SRA |
SRP152284 |
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