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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 08, 2009 |
| Title |
Gyk female heterozygous liver expression vs C57Bl/6J female wildtype control |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
A major task in dissecting the genetics of complex traits is to identify causal genes for disease phenotypes. We previously developed a method to infer causal relationships among genes through the integration of DNA variation, gene transcription, and phenotypic information. Here we validated our method through the characterization of transgenic and knockout mouse models of candidate genes that were predicted to be causal for abdominal obesity. Perturbation of eight out of the nine genes, with Gas7, Me1 and Gpx3 being novel, resulted in significant changes in obesity related traits. Liver expression signatures revealed alterations in common metabolic pathways and networks contributing to abdominal obesity and overlapped with a macrophage-enriched metabolic network module that is highly associated with metabolic traits in mice and humans. Integration of gene expression in the design and analysis of traditional F2 intercross studies allows high confidence prediction of causal genes, and identification of involved pathways and networks.
Keywords: Obesity study in knockout animals
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| Overall design |
A total of 10 mice were used for this study (5 Gyk heterozygotes and 5 B6 wildtype). The gene expression data is from the livers of 23 week old female wildtype and transgenic mice. These mice were fed a 4% fat chow diet until 11 weeks of age and then were fed a 6% fat chow diet until sacrifice at 23 weeks of age.
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| Contributor(s) |
Yang X, Deignan J, Qi H, Zhu J, Qian S, Torosyan G, Majid S, Falkard B, Kleinhanz RR, Karlsson J, Castellani LW, Mumick S, Wang K, Lamb JR, Xia T, Coon M, Zhang C, Estrada-Smith D, Farber CR, Wang SS, Van Nas A, Ghazalpour A, Zhang B, MacNeil DJ, Reitman ML, Dipple K, Mehrabian M, Lum PY, Shadt EE, Lusis AJ, Drake TA |
| Citation(s) |
19270708 |
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| Submission date |
Jul 03, 2008 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Josh Deignan |
| E-mail(s) |
jdeignan@mednet.ucla.edu
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| Organization name |
UCLA
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| Department |
Cardiology
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| Lab |
Aldons J. Lusis
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| Street address |
675 Charles E Young Dr South, MRL 3220
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90024 |
| Country |
USA |
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| Platforms (1) |
| GPL4865 |
Sentrix MouseRef-8 Expression BeadChip (Target ID) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE12000 |
Obesity study in transgenic and knockout animals |
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| Relations |
| BioProject |
PRJNA109241 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11998_GykTXT_gene_profile_RAW.txt |
10.2 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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