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| Status |
Public on Mar 01, 2010 |
| Title |
Gene expression of cytosolic glutamine synthetase 1;1 knock-out mutant rice |
| Platform organism |
Oryza sativa Japonica Group |
| Sample organism |
Oryza sativa |
| Experiment type |
Expression profiling by array
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| Summary |
Rice grown in paddy fields prefers to use ammonium ions as a major source of inorganic nitrogen. Glutamine synthetase (GS) catalyzes the conversion of ammonium ions to glutamine. In three cytosolic GS in rice, OsGS1;1 has the critical role for normal growth and grain filling. To understand a role of GS1;1, we performed transcriptional profiling of wild type Nipponbare and GS1;1 mutant plants in seedling using the Agilent Rice Oligo Microarray.
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| Overall design |
organisms: Oryza sativa L. cv. Nipponbare
mutant: GS1;1 mutant rice (Tos17 insertion NC2373)
growth substrates: The germinated seedlings were grown hydroponically in one-fourth concentration of the hydroponic culture solution with 1.0 mM NH4Cl as nitrogen source. The culture solution was replaced as new one every morning at 10:00 am.
age: 18 days after sowing
tissue: 4th leaf blade derived from 4 plants
tissue: 4th leaf sheath derived from 4 plants
tissue: roots derived from a plant
WT vs. GS1;1 mutant rice
hybridization : two dye method
hybridization: Dye-swap experiment
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| Contributor(s) |
Tabuchi M, Yamaya T |
| Citation missing |
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| |
| Submission date |
Mar 05, 2009 |
| Last update date |
Dec 06, 2012 |
| Contact name |
Tomoyuki Yamaya |
| E-mail(s) |
tyamaya@biochem.tohoku.ac.jp
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| Organization name |
Tohoku University
|
| Department |
Gladuate School of Agricultural Science
|
| Street address |
1-1Tsutsumidori-Amamiyamachi, Aoba-ku
|
| City |
Sendai |
| ZIP/Postal code |
981-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL892 |
Agilent-012106 Rice Oligo Microarray G4138A (Feature Number version) |
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| Samples (6)
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| GSM377898 |
WT rice leaf blade vs. GS1;1 mutant rice leaf blade |
| GSM377899 |
WT rice leaf blade vs. GS1;1 mutant rice leaf blade, dye-swap |
| GSM377900 |
WT rice leaf sheath vs. GS1;1 mutant rice leaf sheath |
| GSM377901 |
WT rice leaf sheath vs. GS1;1 mutant rice leaf sheath, dye-swap |
| GSM377902 |
WT rice root vs. GS1;1 mutant rice root |
| GSM377903 |
WT rice root vs. GS1;1 mutant rice root, dye-swap |
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| Relations |
| BioProject |
PRJNA114877 |
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