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| Status |
Public on Sep 28, 2021 |
| Title |
Pregnancy-activated fibroblasts of the mouse mammary gland |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The mouse mammary gland undergoes severe morphological changes during each pregnancy cycle. These are controlled by epithelial as well as stromal factors, including fibroblasts. This project aimed to identify factors that are expressed in mammary fibroblasts during early pregnancy (day3) when the first morphological changes become microscopically visible.
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| Overall design |
Fibroblasts were isolated from three individual 12-13 weeks old mice (2nd, 3rd, and 4th gland) 3 days after plug formation, as well as from three virgin litter-mates as decribed previously (Ibrahim et al., 2017). After RNA isolation and RNA amplification, labelled cDNA was hybridised to Illumina Whole-genome mouse microarrays
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| |
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| Contributor(s) |
Ibrahim AM, Morris JS, Stein T |
| Citation(s) |
34565423 |
| |
| Submission date |
Feb 24, 2021 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Torsten Stein |
| E-mail(s) |
Torsten.Stein@glasgow.ac.uk
|
| Organization name |
University of Glasgow
|
| Department |
School of Medicine
|
| Lab |
Mammary Gland Morphogenesis and Breast Cancer
|
| Street address |
College of MVLS
|
| City |
Glasgow |
| ZIP/Postal code |
G12 8QQ |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL17543 |
Illumina MouseWG-6 v2.0 R2 expression beadchip |
|
| Samples (6)
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| GSM5105150 |
day3-pregnant mouse mammary gland fibroblasts (non-cultured) replicate 1 |
| GSM5105151 |
day3-pregnant mouse mammary gland fibroblasts (non-cultured) replicate 2 |
| GSM5105152 |
day3-pregnant mouse mammary gland fibroblasts (non-cultured) replicate 3 |
| GSM5105153 |
age-matched virgin mouse mammary gland fibroblasts (non-cultured) replicate 1 |
| GSM5105154 |
age-matched virgin mouse mammary gland fibroblasts (non-cultured) replicate 2 |
| GSM5105155 |
age-matched virgin mouse mammary gland fibroblasts (non-cultured) replicate 3 |
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| Relations |
| BioProject |
PRJNA704654 |
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