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| Status |
Public on May 01, 2024 |
| Title |
Mapping of 2'-O-methylation in Trypanosoma brucei small RNAs using RibOxi-seq |
| Organism |
Trypanosoma brucei brucei TREU927 |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
In this study we combined small RNA isolation from post-ribosomal supernatant with RibOxi-seq to identify 2’-O-methylation (Nm) in single nucleotide resolution. Our analysis identified ~49 Nm sites in tRNA, snRNAs, snoRNAs, 7SL RNA and vtRNA.
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| Overall design |
Trypanosoma brucei procyclic form was used to extract small RNAs. The RNA was then underwent RibOxi-seq for 2'-O-methylation profiling protocol with modifications
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| Contributor(s) |
Zhu Y, Rajan KS, Michaeli S, Holley C |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 01, 2021 |
| Last update date |
May 01, 2024 |
| Contact name |
Christopher L Holley |
| Organization name |
Duke University
|
| Street address |
2301 Erwin Rd
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27705 |
| Country |
USA |
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| Platforms (1) |
| GPL30921 |
Illumina NextSeq 500 (Trypanosoma brucei brucei TREU927) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA776909 |
| SRA |
SRP344110 |
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