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| Status |
Public on Mar 05, 2022 |
| Title |
The Drosophila BEAF Insulator Protein Interacts with the Polybromo Subunit of the PBAP Chromatin Remodeling Complex [PRO-seq] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
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| Summary |
We examine the effect of this interaction on gene expression and chromatin structure using precision run-on sequencing (PRO-seq) and micrococcal nuclease sequencing (MNase-seq) after RNAi-mediated knockdown in cultured S2 cells.
We examine the effect of the interaction between BEAF and polybromo on gene expression and chromatin structure using precision run-on sequencing (PRO-seq) and micrococcal nuclease sequencing (MNase-seq) after RNAi-mediated knockdown in cultured S2 cells.
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| Overall design |
Two biological replicates were sampled for each RNAi treatment, LacZ, BEAF, and polybromo. Nuclei were isolated and used for library preparation.
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| Contributor(s) |
McKowen JK, Avva SV, Maharjan M, Duarte FM, Tome JM, Wood JL, Negedu S, Dong Y, Lis JT, Hart CM |
| Citation(s) |
36029240 |
| |
| Submission date |
Feb 28, 2022 |
| Last update date |
Nov 29, 2022 |
| Contact name |
Craig Hart |
| E-mail(s) |
keller.mckowen@gmail.com, chart4@lsu.edu
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| Phone |
225-578-7389
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| Organization name |
Louisiana State University
|
| Department |
Biological Sciences
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| Lab |
Hart
|
| Street address |
Life Science Building, LSU
|
| City |
Baton Rouge |
| State/province |
Louisiana |
| ZIP/Postal code |
70808 |
| Country |
USA |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE197584 |
The Drosophila BEAF Insulator Protein Interacts with the Polybromo Subunit of the PBAP Chromatin Remodeling Complex |
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| Relations |
| BioProject |
PRJNA811127 |
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