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| Status |
Public on Jul 18, 2022 |
| Title |
Protocol for Mapping Protein-DNA Interactions with Directed Methylation with Long-read Sequecing (DiMeLo-seq) |
| Organisms |
Drosophila melanogaster; Homo sapiens |
| Experiment type |
Other
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| Summary |
DiMeLo-seq uses a protein A Hia5 fusion to direct adenine methylation at specific regions of the genome using antibodies for chromatin-bound factors. Adenine and cytosine methylation are then read out by methylation-sensitive long-read sequencing.
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| Overall design |
DiMeLo-seq is performed in situ on H3K27ac, H3K27me3, and H3K4me3 in GM12878 cells. DiMeLo-seq is also performed in situ on H3K9me3 in D. melanogaster embryos.
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| Contributor(s) |
Maslan A, s Altemose N, Streets A |
| Citation missing |
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| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HG012383 |
Methods for Mapping Genetic Regulatory Elements in Single Cells and Single Molecules |
UNIVERSITY OF CALIFORNIA BERKELEY |
Aaron Streets |
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| BioProject |
PRJNA855257 |
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| Submission date |
Jul 13, 2022 |
| Last update date |
Jul 20, 2022 |
| Contact name |
Aaron Streets |
| E-mail(s) |
astreets@berkeley.edu
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| Organization name |
University of California, Berkeley
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| Department |
Bioengineering
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| Street address |
Stanley Hall
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (2) |
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| Samples (4)
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