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| Status |
Public on Jul 15, 2022 |
| Title |
Expression data from 1. leucine deprivation and 2. ATF4 overexpression on HepG2 cells |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
The mechanistic target of rapamycin complex 1 (mTORC1) is involved in nutrient-induced signaling and is a master regulator of cell growth and metabolism. Amino acid-deficient conditions affect mTORC1 activity; however, its upstream regulators warrant further investigation. MicroRNAs are key regulators of nutrient-related responses; therefore, the present study aimed to assess the leucine starvation-induced microRNA profile and its impact on mTORC1 activity. Transcriptome analysis of human hepatocellular carcinoma cells (HepG2) under leucine deprivation revealed that hsa-miR-663a and hsa-miR-1469 were altered in a transcription factor 4-dependent manner. Overexpression of these microRNAs induced phosphorylation of the ribosomal protein S6 kinase beta-1, a mTORC1 downstream target. Furthermore, hsa-miR-663a downregulated proline-rich Akt1 substrate of 40 kDa (PRAS40), one of the mTORC1 components. In summary, this study provides new insights into the regulatory role of microRNAs in amino acid metabolism and demonstrate alterations in microRNA profile under leucine deprivation in human hepatocytes.
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| Overall design |
Leucine deprivation and ATF4 overexpression treated HepG2 cells were used for RNA extraction and hybridization on Affymetrix microarrays.
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| Contributor(s) |
Yamamura J, Ma S, Jia H, Kato H |
| Citation(s) |
35990342 |
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| Submission date |
Jul 14, 2022 |
| Last update date |
Aug 26, 2022 |
| Contact name |
Sihui Ma |
| E-mail(s) |
masihui@toki.waseda.jp
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| Organization name |
The university of Tokyo
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| Street address |
Yayoi 1-1-1
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| City |
Tokyo |
| ZIP/Postal code |
1130032 |
| Country |
Japan |
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| Platforms (1) |
| GPL16384 |
[miRNA-3] Affymetrix Multispecies miRNA-3 Array |
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| Samples (6)
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| GSM6339335 |
HepG2 cell, control sample for leucine deprivation condition, bio rep1 |
| GSM6339336 |
HepG2 cell, control sample for leucine deprivation condition, bio rep2 |
| GSM6339337 |
HepG2 cell, leucine deprivation sample under leucine deprivation condition, bio rep1 |
| GSM6339338 |
HepG2 cell, leucine deprivation sample under leucine deprivation condition, bio rep2 |
| GSM6339339 |
HepG2 cell, mock sample for ATF4 overexpression condition |
| GSM6339340 |
HepG2 cell, ATF4 transfect sample for ATF4 overexpression condition |
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| Relations |
| BioProject |
PRJNA858810 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE208228_RAW.tar |
27.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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