|
| Status |
Public on Apr 09, 2010 |
| Title |
Expression data from undifferentiated and induced human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Due to their somatic cell origin, human induced pluripotent stem cells (HiPSCs) are assumed to carry a normal diploid genome, and adaptive chromosomal aberrations have not been fully evaluated. Here, we analyzed the chromosomal integrity of 66 HiPSC and 38 human embryonic stem cell (HESC) samples from 18 different studies by global gene expression meta-analysis. We report identification of a substantial number of cell lines carrying full and partial chromosomal aberrations, half of which were validated at the DNA level. Several aberrations resulted from culture adaptation, and others are suspected to originate from the parent somatic cell. Our classification revealed a third type of aneuploidy already evident in early passage HiPSCs, suggesting considerable selective pressure during the reprogramming process. The analysis indicated high incidence of chromosome 12 duplications, resulting in significant enrichment for cell cycle related genes. Such aneuploidy may limit the differentiation capacity and increase the tumorigenicity of HiPSCs.
This SuperSeries is composed of the SubSeries listed below.
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| |
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| Overall design |
Refer to individual Series
|
| |
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| Citation(s) |
20887957 |
| |
| Submission date |
Apr 08, 2010 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Uri Ben-David |
| E-mail(s) |
uri.bendavid@gmail.com
|
| Phone |
97226585185
|
| Organization name |
Hebrew University
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty
|
| Street address |
Givat-Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
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| Platforms (2) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (11)
|
| GSM531015 |
undifferentiated induced pluripotent stem cell clone 3975.2 |
| GSM531016 |
undifferentiated induced pluripotent stem cell clone 3975.4 |
| GSM531017 |
undifferentiated induced pluripotent stem cell clone IMR9.4 |
| GSM531018 |
undifferentiated induced pluripotent stem cell clone hIPS18_P10_CM |
| GSM531019 |
undifferentiated H9 Human embryonic stem cells replicate 1 |
| GSM531020 |
undifferentiated H9 Human embryonic stem cells replicate 2 |
| GSM531021 |
undifferentiated H9 Human embryonic stem cells replicate 3 |
| GSM531022 |
undifferentiated BJ-TERT derived induced pluripotent stem cell line 28 replicate 2 |
| GSM531023 |
undifferentiated BJ-TERT derived induced pluripotent stem cell line 28 replicate 1 |
| GSM531024 |
undifferentiated primary foreskin fibroblast derived induced pluripotent stem cell line 1 |
| GSM531025 |
undifferentiated BJ-TERT derived induced pluripotent stem cell line 29 |
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| This SuperSeries is composed of the following SubSeries: |
| GSE21243 |
Expression data from undifferentiated human induced pluripotent stem cells |
| GSE21244 |
Expression data from undifferentiated human pluripotent stem cells |
|
| Relations |
| BioProject |
PRJNA126271 |
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