|
| Status |
Public on Apr 09, 2010 |
| Title |
undifferentiated primary foreskin fibroblast derived induced pluripotent stem cell line 1 |
| Sample type |
RNA |
| |
|
| Source name |
undifferentiated primary foreskin fibroblast derived induced pluripotent stem cell line 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Undifferentiated human induced pluripotent stem cells
genotype: 46(X,Y)
age: passage 5
cell line: primary foreskin fibroblast derived induced pluripotent stem cell line 1
|
| Treatment protocol |
total RNA was isolated from undifferentiated pluripotent stem cells grown in standard HESC growth conditions on mouse embryonic fibroblast feeder layer.
|
| Growth protocol |
standard HESC growth conditions as described in Pick et al. (2009). iPSC lines were induced by infection with a combinationn of the human OCT4, SOX2, MYC and KLF4 genes packaged in pMXs retroviral vectors, as described in Pick et al. (2009)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tri reagent
|
| Label |
biotin
|
| Label protocol |
300ng total RNA, Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
GeneChip scanner 3000 7G, GeneChip Operating Software v1.4
|
| Description |
Gene expression data from undifferentiated primary foreskin fibroblast derived induced pluripotent stem cell line 1
|
| Data processing |
Affymetrix Expression Console RMA
|
| |
|
| Submission date |
Apr 07, 2010 |
| Last update date |
Apr 08, 2010 |
| Contact name |
Uri Ben-David |
| E-mail(s) |
uri.bendavid@gmail.com
|
| Phone |
97226585185
|
| Organization name |
Hebrew University
|
| Department |
Genetics
|
| Lab |
Nissim Benvenisty
|
| Street address |
Givat-Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE21244 |
Expression data from undifferentiated human pluripotent stem cells |
| GSE21262 |
Expression data from undifferentiated and induced human pluripotent stem cells |
|
