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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 19, 2022 |
| Title |
Autonomous rhythmic activity in glioma networks drives brain tumor growth |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Glioblastoma cell networks harbor a plastic population of highly active glioblastoma cells that display rhythmic Ca2+ oscillations and are particularly connected to others. Targeting the autonomous rhythmic activity of periodic tumor cells by pharmacological interference with the potassium channel KCa3.1 strongly compromised global network communication. This led to a marked reduction of tumor cell viability within the entire network, reduced tumor growth in mice, and prolonged animal survival. The dependency of glioblastoma networks on periodic Ca2+ activity generates a vulnerability that can be exploited for the development of novel therapies, with KCa3.1 inhibiting drugs as one example.
To investigate the molecular mechanism of KCa3.1 blockade, we exposed glioblastoma cells to Senicapoc, a selective inhinitor of the KCa3.1 channel, and performed gene expression profiling analysis using data obtained from RNA-Seq.
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for S24 cells and its Senicapoc treated derivates.
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| Contributor(s) |
Hausmann D, Hoffmann DC, Venkataramani V, Jung E, Horschitz S, Tetzlaff SK, Jabali A, Hai L, Kessler T, Azorin DD, Weil S, Kourtesakis A, Sievers P, Habel A, Breckwoldt MO, Karreman MA, Ratliff M, Messmer JM, Yang Y, Reyhan E, Wendler S, Löb C, Mayer C, Figarella K, Osswald M, Solecki G, Sahm F, Garaschuk O, Kuner T, Koch P, Schlesner M, Wick W, Winkler F |
| Citation(s) |
36517594 |
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| Submission date |
Oct 12, 2022 |
| Last update date |
Jan 07, 2023 |
| Contact name |
Frank Winkler |
| E-mail(s) |
Frank.Winkler@med.uni-heidelberg.de
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| Organization name |
University Hospital Heidelberg, Heidelberg
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| Department |
Neurology Clinic and National Center for Tumor Diseases
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| Street address |
Im Neuenheimer Feld 400
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| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA889854 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE215365_S24_Control_Senicapoc_log2FPKM.txt.gz |
646.0 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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