|
| Status |
Public on Oct 19, 2022 |
| Title |
S24_line_Semicapoc_Rep_1 |
| Sample type |
SRA |
| |
|
| Source name |
S24
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: S24
cell type: Patient derived glioblastoma cell line
genotype: WT
treatment: Senicapoc
time: Day 2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy Mini kit (Qiagen). 1.0 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using Illumina TruSeq Stranded RNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
The RNA sequencing data was aligned against human genome GRCh38 by STAR (v.2.5.3a)
The reads mapped to multiple loci were filtered
The gene counts were quantified against GENCODE annotation (v.32) by the featureCounts function from Subread (v.1.5.3) with default setting
Assembly: Human genome GRCh38
Supplementary files format and content: Tab-delimited text files include log2(FPKM) values for each Sample
|
| |
|
| Submission date |
Oct 12, 2022 |
| Last update date |
Oct 19, 2022 |
| Contact name |
Frank Winkler |
| E-mail(s) |
Frank.Winkler@med.uni-heidelberg.de
|
| Organization name |
University Hospital Heidelberg, Heidelberg
|
| Department |
Neurology Clinic and National Center for Tumor Diseases
|
| Street address |
Im Neuenheimer Feld 400
|
| City |
Heidelberg |
| ZIP/Postal code |
69120 |
| Country |
Germany |
| |
|
| Platform ID |
GPL24676 |
| Series (1) |
| GSE215365 |
Autonomous rhythmic activity in glioma networks drives brain tumor growth |
|
| Relations |
| BioSample |
SAMN31262337 |
| SRA |
SRX17869973 |
