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| Status |
Public on Feb 27, 2023 |
| Title |
Acidosis activates the Nrf2-pathway in renal proximal tubule-derived cells through a crosstalk with renal fibroblasts |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Crosstalk of renal epithelial cells with interstitial fibroblasts plays an important role in kidney pathophysiology. A former study showed that crosstalk between renal epithelial cells and renal fibroblasts protects against acidosis-induced damage. In order to gain further mechanistic insight into this crosstalk we investigated the effect of acidosis on the transcriptome of renal epithelial cells (NRK-52E) and renal fibroblasts (NRK-49F) in co-culture by RNASeq, bioinformatics analysis and experimental validation.
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| Overall design |
NRK-52E (proximal tubule-derived epithelial cells) and NRK-49F (kidney fibroblasts) were subjected to acidic conditions, while being co-cultivated.
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| Contributor(s) |
Schulz M, Dubourg V, Nolze A, Kopf M, Schwerdt G, Gekle M |
| Citation(s) |
36829971 |
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| Submission date |
Nov 23, 2022 |
| Last update date |
Mar 09, 2023 |
| Contact name |
Virginie Dubourg |
| Organization name |
Martin Luther University - Halle-Wittenberg
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| Department |
Julius Bernstein Institute for Physiology
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| Street address |
Magdeburger Str. 6
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| City |
Halle (Saale) |
| ZIP/Postal code |
06112 |
| Country |
Germany |
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| Platforms (1) |
| GPL25947 |
Illumina NovaSeq 6000 (Rattus norvegicus) |
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| Samples (16)
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| GSM6753436 |
N4 NRK-52E cells, pH6 |
| GSM6753437 |
N5 NRK-52E cells, pH7 |
| GSM6753438 |
N6 NRK-52E cells, pH7 |
| GSM6753439 |
N7 NRK-52E cells, pH6 |
| GSM6753440 |
N8 NRK-52E cells, pH6 |
| GSM6753441 |
N9 NRK-49F cells, pH7 |
| GSM6753442 |
N10 NRK-49F cells, pH7 |
| GSM6753443 |
N11 NRK-49F cells, pH6 |
| GSM6753444 |
N12 NRK-49F cells, pH6 |
| GSM6753445 |
N13 NRK-49F cells, pH7 |
| GSM6753446 |
N14 NRK-49F cells, pH7 |
| GSM6753447 |
N15 NRK-49F cells, pH6 |
| GSM6753448 |
N16 NRK-49F cells, pH6 |
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| Relations |
| BioProject |
PRJNA904711 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE218626_annotatedCounts.csv.gz |
824.4 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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