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Status |
Public on Feb 27, 2023 |
Title |
N8 NRK-52E cells, pH6 |
Sample type |
SRA |
|
|
Source name |
NRK-52E
|
Organism |
Rattus norvegicus |
Characteristics |
cell line: NRK-52E cell type: Epithelial treatment: pH6
|
Treatment protocol |
Cells were synchronized for 24h with medium without FCS. The incubation with pH 7.4 or 6.0 was then started and was maintained for 48h
|
Growth protocol |
NRK-52E and NRK-49F were cultivated with DMEM medium (5% FCS, 1.5g/L NaHCO3), at 37°C, 5% CO2. For co-culture conditions, NRK-49F were directly sed in wells of 6-well plates, while NRK-52E were grown on permeable filter inserts that were embed onto the 6-well plates with the NRK-49F.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated with BluZol Reagent, following the manufacturer's instructions. Eventual gDNA contaminations were removed using Turbo DNAse (ambion, Life Technologies). polyA enrichment (performed by Novogene Europe)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Data processing |
Read alignment (HISAT2 2.1.0) Read counting (featureCounts - 2.0.0) Annotation with biomaRt and Ensembl 99 Assembly: rn6 Supplementary files format and content: .csv file including annotation raw counts (output of featureCounts) for each sample
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|
|
Submission date |
Nov 23, 2022 |
Last update date |
Feb 27, 2023 |
Contact name |
Virginie Dubourg |
Organization name |
Martin Luther University - Halle-Wittenberg
|
Department |
Julius Bernstein Institute for Physiology
|
Street address |
Magdeburger Str. 6
|
City |
Halle (Saale) |
ZIP/Postal code |
06112 |
Country |
Germany |
|
|
Platform ID |
GPL25947 |
Series (1) |
GSE218626 |
Acidosis activates the Nrf2-pathway in renal proximal tubule-derived cells through a crosstalk with renal fibroblasts |
|
Relations |
BioSample |
SAMN31851373 |
SRA |
SRX18360544 |