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Series GSE230770 Query DataSets for GSE230770
Status Public on Mar 31, 2024
Title Clinicopathological and molecular landscape of 5-year IDH-wild-type glioblastoma survivors: a multicentric retrospective study
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary Background: Less than 5% of glioblastoma multiforme (GBM) patients survive more than 5-years (long-term survivors, LTS). Nevertheless, the molecular fingerprint of LTS remains uncharted. We aimed to characterize LTS by clinicopathological assessment, DNA methylation (DNAm)/Copy Number Variation (CNV) and mutation profiling. Methods: This multicentric project proposed by Alliance Against Cancer was coordinated by Fondazione IRCCS Istituto Neurologico Carlo Besta. One-hundred forty-two LTS patients were screened, and samples centrally reviewed; 95 patients were enrolled. DNAm profiles and sequencing were performed on 27 LTS and 24 standard survival GBM (STS). Results: We focused on 73 IDHwt-LTS. All patients underwent at least partial resection, followed by Stupp protocol (48/70). The median time to progression was 43 months (4-186), and almost all received a second treatment. Morphological and routine diagnostic molecular features of LTS did not deviate from the classic findings. MGMT promoter methylation was detected in 92% of cases. No significant differences were observed between LTS and STS in terms of prevalence of mutated genes, with TP53 as the most commonly mutated gene (26% of all samples). DNAm showed a more heterogeneous group for LTS, with several cases classified as pediatric high-grade glioma and peculiar CNVs. We identified 18 differentially methylated regions, 4 associated with survival probability (NR2F2, MME, WDR66 and ENPP4). Methylation levels of individual probes in IGFBP3 gene and corresponding protein expression showed a significant correlation with survival. Conclusions: We found no significant different morphology nor mutational profile of LTS. DNAm confirmed as a valuable tool for GBM classification. No specific episignature was detected, but methylation levels of specific genes had prognostic value in LTS.
 
Overall design 27 LTS (long-term survival) glioblastoma patients were compared to 24 standard survival (control) glioblastoma patients
Web link https://doi.org/10.1016/j.canlet.2024.216711
 
Contributor(s) Miele E, Patrizi S, Ciolfi A, Pedace L, Barresi S, Mastronuzzi A, Tartaglia M, Locatelli F
Citation(s) 38423245
Submission date Apr 27, 2023
Last update date Jul 01, 2024
Contact name Evelina Miele
E-mail(s) evelina.miele@opbg.net
Organization name Ospedale Pediatrico Bambino Gesù
Department Oncoematologia
Street address Piazza di Sant'Onofrio 4
City Rome
ZIP/Postal code 00165
Country Italy
 
Platforms (1)
GPL21145 Infinium MethylationEPIC
Samples (51)
GSM7234039 Standard survival (control) glioblastoma FFPE sample 1
GSM7234040 Standard survival (control) glioblastoma FFPE sample 2
GSM7234041 Standard survival (control) glioblastoma FFPE sample 3
Relations
BioProject PRJNA962547

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE230770_RAW.tar 875.0 Mb (http)(custom) TAR (of IDAT)
Processed data included within Sample table
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