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| Status |
Public on Jul 31, 2024 |
| Title |
Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma [blood_vessels_PDXs_tumours_bulk] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome profile of Gr3-MB cells located distal and proximal to blood vessels in the PDXs.
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| Overall design |
The experiment was performed as described previously with modifications (Kumar et al., 2019). Briefly, Group 3 medulloblastoma PDXs (Med-411FH, Med-211FH, BT2019051) were engrafted into NSG mice. Once the animal reaches humane endpoint, Hoechst 33342 (2.5mg/kg body weight) was injected through the tail vein and after 15 min the tumor was resected. Tumors cells were dissociated using enzyme-free cell dissociation solution (Millipore # S014C) and the single cell suspension was passed through a 70 micron cell strainer. The tumor cells are sorted based on Hoechst uptake using BD FACSAria III cell sorter.
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| Contributor(s) |
Taylor MD |
| Citation(s) |
38971152 |
| |
| Submission date |
Jul 14, 2023 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Vikas Patil |
| Organization name |
Princess Margaret Cancer Center
|
| Lab |
MacFeeters-Hamilton Centre for Neuro-Oncology Research
|
| Street address |
610 University Ave.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M3A3B3 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (10)
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| This SubSeries is part of SuperSeries: |
| GSE237381 |
Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma |
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| Relations |
| BioProject |
PRJNA994849 |
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