|
| Status |
Public on Jul 31, 2024 |
| Title |
Med_211hc_pos |
| Sample type |
SRA |
| |
|
| Source name |
Med_211
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Med_211
cell type: Medulloblastoma
treatment: Hoechst 33342
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA was extracted with RNeasy kit (Qiagen, 74004) as per manufacturers instructions.
RNA converted into cDNA library using TruSeq Stranded Total RNA kit (Illumina) using manufacturers instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
Med_211hc_pos
|
| Data processing |
Quality of reads assessed by FastQC tool.
The reads were aligned to the human reference genome using Star aligner.
RNA-Seq reads were counted using HtSeq and differnetial expression was analysed by DEseq2.
Assembly: GRCh38
Supplementary files format and content: count matrix (txt.file)
|
| |
|
| Submission date |
Jul 14, 2023 |
| Last update date |
Jul 31, 2024 |
| Contact name |
Vikas Patil |
| Organization name |
Princess Margaret Cancer Center
|
| Lab |
MacFeeters-Hamilton Centre for Neuro-Oncology Research
|
| Street address |
610 University Ave.
|
| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M3A3B3 |
| Country |
Canada |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE237379 |
Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma [blood_vessels_PDXs_tumours_bulk] |
| GSE237381 |
Prtg+ve Rhombic Lip Ventricular Zone Stem Cells Initiate and Maintain Group 3 Medulloblastoma |
|
| Relations |
| BioSample |
SAMN36451433 |
| SRA |
SRX21023757 |
