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| Status |
Public on Oct 05, 2012 |
| Title |
Identification of genes regulated by XOct-25 overexpression in Xenopus ectoderm |
| Organism |
Xenopus laevis |
| Experiment type |
Expression profiling by array
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| Summary |
The Xenopus POU class V transcription factor XOct-25 has been shown to inhibit BMP-dependent epidermal differentiation and promote neural induction in the ectoderm during early embryogenesis. In order to identify genes that act downstream of XOct-25, transcriptional profile of Xenopus ectodermal cells overexpressing XOct-25 was compared with control cells by using a DNA microarray method.
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| Overall design |
Two independent experiments. Each experiment contains ectodermal cells overexpressing XOct-25 and corresponding control cells. Xenopus embryos were injected at the 8-cell stage with mRNA encoding GR-XOct-25, a hormone-inducible version of XOct-25. Explants from stage 9 embryos were treated with or without dexamethazone (DEX) until the sibling embryos reached stage 10.5, when they were used for RNA extraction. The explants cultured without DEX was used as a control sample.
biological replicates: Sample name XOct-exp 1, Sample name XOct-exp 2
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| Contributor(s) |
Takebayashi-Suzuki K, Kitayama A, Terasaka-Iioka C, Ueno N, Suzuki A |
| Citation(s) |
21958745 |
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| Submission date |
Oct 06, 2010 |
| Last update date |
Jul 31, 2013 |
| Contact name |
Atsushi Suzuki |
| Organization name |
Hiroshima University
|
| Department |
Graduate School of Science
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| Lab |
Institute for Amphibian Biology
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| Street address |
Kagamiyama 1-3-1
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| City |
Higashi-hiroshima |
| State/province |
Hiroshima |
| ZIP/Postal code |
739-8526 |
| Country |
Japan |
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| Platforms (1) |
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| Samples (2) |
| GSM605600 |
XOct-25 overexpression-experiment 1 |
| GSM605601 |
XOct-25 overexpression-experiment 2 |
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| Relations |
| BioProject |
PRJNA132629 |
