|
Status |
Public on Oct 19, 2005 |
Title |
HCaRG-9 vs NEO-1 |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
|
Summary |
HEK293 cells were transfected with control plasmid (pcDNAI/Neo;Invitrogen) or with the plasmid encoding HCaRG. Stable transfectants were synchronized and grown in the presence of 10% FBS for 48 h. Total RNAs were purified with the mini RNeasy kit (Qiagen).
Chips HG-U133A: - Labeling protocol: Enzo BioArray HighYield RNA Transcript Labeling Kit. - Hybridization: According to the manufacturer's protocol (Affymetrix). - Scanner: GeneArray 2500. - Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500.
Chips HG-U133 Plus 2.0: - Labeling protocol: GeneChip IVT Labeling Kit. - Hybridization: According to the manufacturer's protocol (Affymetrix). - Scanner: GeneChip Scanner 3000. - Normalization: Employing GCOS software, chips were normalized using all probe sets scaling option and target signal at 500. Keywords: parallel sample
|
|
|
|
|
Contributor(s) |
El Hader C, Tremblay S, Solban N, Gingras D, Béliveau R, Orlov S, Hamet P, Tremblay J |
Citation(s) |
16033922 |
|
Submission date |
Apr 21, 2005 |
Last update date |
Mar 25, 2019 |
Contact name |
Johanne Tremblay |
E-mail(s) |
johanne.tremblay@umontreal.ca
|
Phone |
514-890-8000-12721
|
Organization name |
CHUM Research Center, Hotel-Dieu
|
Street address |
|
City |
Montreal |
State/province |
QUE |
ZIP/Postal code |
H2W 1T8 |
Country |
Canada |
|
|
Platforms (2) |
GPL96 |
[HG-U133A] Affymetrix Human Genome U133A Array |
GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
|
Samples (6)
|
|
Relations |
BioProject |
PRJNA92165 |