HEK293 cells were transfected with pcDNAI/Neo (Invitrogen) as control plasmid. Stable transfectants were synchronized and grown in the presence of 10% FBS for 48 h. Total RNAs were purified with the mini RNeasy kit (Qiagen).
Data processing
Chip was normalized using all probe sets scaling option and target signal at 500 (GCOS1.2).
