|
| Status |
Public on May 06, 2024 |
| Title |
Antagonistic conflict between transposon-encoded introns and guide RNAs (RIP-Seq) |
| Organism |
Escherichia coli |
| Experiment type |
Other
|
| Summary |
TnpB nucleases represent the evolutionary precursors to CRISPR-Cas12 and are widespread in all domains of life. IS605-family TnpB homologs function in bacteria as programmable RNA-guided homing endonucleases driving transposon maintenance through DSB-stimulated homologous recombination. Here we uncover molecular mechanisms of transposition lifecycle of IS607-family elements that, remarkably, also encode group I introns. We discover molecular features for a candidate ‘IStron’ from Clostridium botulinum that allow the element to carefully control the relative levels of spliced products versus functional guide RNAs. Our results suggest that IStron transcripts have evolved a sensitive equilibrium to balance competing and mutually exclusive activities that promote transposon maintenance while limiting adverse fitness costs on the host. Collectively, this work highlights molecular innovation in the multi-functional utility of transposon-encoded noncoding RNAs.
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| |
|
| Overall design |
E. coli RIP-seq profiles for CboTnpB.
|
| |
|
| Contributor(s) |
Žedaveinytė R, Meers C, Le HC, Mortman EE, Tang S, Lampe GD, Pesari SR, Gelsinger DR, Wiegand T, Sternberg SH |
| Citation(s) |
38991068 |
| |
| Submission date |
Mar 11, 2024 |
| Last update date |
Aug 05, 2024 |
| Contact name |
Samuel Henry Sternberg |
| E-mail(s) |
shsternberg@gmail.com
|
| Phone |
717-475-3658
|
| Organization name |
Columbia University
|
| Department |
Biochemistry and Molecular Biophysics
|
| Lab |
Sternberg Lab
|
| Street address |
701 W. 168th Street, HHSC 726
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21222 |
Illumina NextSeq 500 (Escherichia coli) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE261344 |
Antagonistic conflict between transposon-encoded introns and guide RNAs |
|
| Relations |
| BioProject |
PRJNA1086529 |
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