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| Status |
Public on Sep 15, 2011 |
| Title |
Expression data from lung tumor and stromal cells of KrasTgfbr2 -/- mouse model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Recent data suggests that repression of the Type II TGF-B Receptor (Tgfr2) repression in human lung adenocarcinoma is important for progression from noninvasive to invasive adenocarcinoma. To test this hypothesis in a animal model of non-invasive lung cancer, we generated an inducible, lung specific Tgfbr2 knockout model in the oncogenic Kras mouse.
LSL-KrasG12D positive mice were simultaneously backcrossed to C57/Bl6 mice and to the Tgfbr2 flox/flox mice. To induce tumors, 100 ul of saline containing 3x10e10 particles of an adenovirus containing the Cre recombinase (Ad.Cre) was administered to each LSL-KrasG12D mouse intra-nasally.
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| Overall design |
We evaluated the tumor microenvironment response to Tgfbr2 deficient tumor cells. We compared lung tumor cell and stromal cell transcriptional profiles from five-week KrasTgfbr2 -/- and nine-week KrasTgfbr2 WT mice. We used mice at these time points to allow comparison of the stromal compartment of similarly advanced tumors.
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| Contributor(s) |
Borczuk AC, Powell CA |
| Citation(s) |
21911454 |
| |
| Submission date |
Mar 04, 2011 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Charles A Powell |
| E-mail(s) |
charles.powell@mssm.edu
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| Fax |
212-876-5519
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| Organization name |
Mount Sinai School of Medicine
|
| Department |
MEDICINE/PULMONARY
|
| Street address |
One Gustave Levy Place, Box 1232
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| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10029 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (14)
|
| GSM685459 |
KrasTgfbr2-/- stroma specimen 111 |
| GSM685460 |
KrasTgfbr2-/- stroma specimen 112 |
| GSM685461 |
KrasTgfbr2-/- stroma specimen 113 |
| GSM685462 |
KrasTgfbr2-/- stroma specimen 114 |
| GSM685463 |
KrasTgfbr2wt lung tumor specimen 101 |
| GSM685464 |
KrasTgfbr2wt lung tumor specimen 102 |
| GSM685465 |
KrasTgfbr2wt lung tumor specimen 103 |
| GSM685466 |
KrasTgfbr2-/- lung tumor specimen 111 |
| GSM685467 |
KrasTgfbr2-/- lung tumor specimen 112 |
| GSM685468 |
KrasTgfbr2-/- lung tumor specimen 113 |
| GSM685469 |
KrasTgfbr2-/- lung tumor specimen 114 |
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| This SubSeries is part of SuperSeries: |
| GSE27719 |
Lung adenocarcinoma invasion and progression |
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| Relations |
| BioProject |
PRJNA141881 |
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