|
| Status |
Public on Nov 06, 2012 |
| Title |
Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration |
| Platform organisms |
Danio rerio; Gallus gallus; Lemur catta; Ateles sp.; Gorilla gorilla; Pan troglodytes; Homo sapiens; Bos taurus; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human betaherpesvirus 5; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Monodelphis domestica; Human gammaherpesvirus 8; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Ambystoma mexicanum |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
The salamander microRNA expression between mid-bud limb regenerating blastemas (17 days post amputation) and non-regenerating stump tissues was compared by microarray analysis.
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| |
|
| Overall design |
LC Sciences arrays: Six paired samples were analyzed: three mid-bud 17dpa blastemas (bl), and three non-regenerating stumps (st). Three arrays were hybridized comparing two paired samples each time. Biological dye-swaps were made by labeling bl samples once with Cy3 and twice with Cy5; st samples were labeled accordingly twice with Cy5 and once with Cy3. Multiple arrays averaged into a single Sample record.
Supplementary files:
GSE29727_LC_MultiArray_SimpleNormalizedData.txt.gz
GSE29727_LC_signal_ratio_mean_SD.txt.gz
GSE29727_LC_t-Test_st-vs-bl.txt.gz
Exiqon arrays: Six paired samples were analyzed: three mid-bud 17dpa blastemas (bl), and three non-regenerating stumps (st). Six arrays were hybridized comparing each sample labeled with Hy3 against a common reference sample made by pooling all the samples and labeling it with Hy5. Multiple arrays not averaged, represented as multiple Sample records.
Supplementary file: GSE29727_Exiqon_matrix_complete.txt
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| |
|
| Contributor(s) |
Holman EC, Crews CM |
| Citation(s) |
23028429 |
| |
| Submission date |
Jun 03, 2011 |
| Last update date |
Feb 05, 2013 |
| Contact name |
Edna C Holman |
| E-mail(s) |
edna96@hotmail.com
|
| Phone |
203-432-6553
|
| URL |
http://www.yale.edu/crews/
|
| Organization name |
Yale University
|
| Department |
Molecular, Cellular and Developmental Biology
|
| Lab |
Craig Crews
|
| Street address |
266 Whitney Ave. KBT-442
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL7724 |
miRCURY LNA microRNA Array, v. 9.2, all organisms |
| GPL13686 |
LC_MRA-1001C_miRHuman_12.0_080930 (miRNA ID version) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA141103 |
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