|
| Status |
Public on Nov 06, 2012 |
| Title |
Blastema_17dpa_rep2 [Exiqon] |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Mid-bud regenerating blastema
|
| Organism |
Ambystoma mexicanum |
| Characteristics |
days post amputation: 17
tissue: Mid-bud regenerating blastema
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
|
| Label |
Hy3
|
| Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit (Outsourced to Exiqon)
|
| |
|
| Channel 2 |
| Source name |
Common Reference
|
| Organism |
Ambystoma mexicanum |
| Characteristics |
sample type: Common Reference (made by pooling all the samples)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extracted using mirVana miRNA Isolation Kit (Ambion) manufacturer's instructions
|
| Label |
Hy5
|
| Label protocol |
The samples were labeled using the miRCURY™ Hy3™/Hy5™ power labeling kit (Outsourced to Exiqon)
|
| |
|
| |
| Hybridization protocol |
The samples were hybridized on the miRCURY™ LNA Array (v.9.2) as per Exiqons protocols.
|
| Scan protocol |
Arrays were scanned as per Exiqons protocols.
|
| Description |
369bl
Biological Replicate 2 of 3
Slide3
Cy3: 1_Exiqon_13768590_S01_Cropped.txt
Cy5: 0_Exiqon_13768590_S01_Cropped.txt
|
| Data processing |
Log ratios between the Hy3/Hy5 have been provided along with the normalized independent Hy3 and Hy5 signals. The quantified signals (background correction) were normalized using the global Lowess (LOcally WEighted Scatterplot Smoothing) regression algorithm.
|
| |
|
| Submission date |
Jun 10, 2011 |
| Last update date |
Nov 06, 2012 |
| Contact name |
Edna C Holman |
| E-mail(s) |
edna96@hotmail.com
|
| Phone |
203-432-6553
|
| URL |
http://www.yale.edu/crews/
|
| Organization name |
Yale University
|
| Department |
Molecular, Cellular and Developmental Biology
|
| Lab |
Craig Crews
|
| Street address |
266 Whitney Ave. KBT-442
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06511 |
| Country |
USA |
| |
|
| Platform ID |
GPL7724 |
| Series (1) |
| GSE29727 |
Microarray Analysis of microRNA Expression during Axolotl Limb Regeneration |
|
