|
| Status |
Public on Jul 29, 2011 |
| Title |
RNAi promotes heterochromatic silencing through replication-coupled release of RNA pol II (ChIP-seq) |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Heterochromatin comprises tightly compacted repetitive regions of eukaryotic chromosomes. The inheritance of heterochromatin through mitosis requires RNA interference (RNAi), which guides histone modification 1 during the DNA replication phase of the cell cycle2. Here, we show that the alternating arrangement of origins of replication and non-coding RNA in pericentromeric heterochromatin results in competition between transcription and replication. Co-transcriptional RNAi releases RNA polymerase II (PolII), allowing completion of DNA replication by the leading strand DNA polymerase, and associated histone modifying enzymes3 which spread heterochromatin with the replication fork. In the absence of RNAi, stalled forks are repaired by homologous recombination without histone modification.
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| Overall design |
RNA PolII ChIP from wild type and dcr1-deleted samples in exponentially growing and HU-arrested cultures.
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| Contributor(s) |
Zaratiegui M, Castel SE, Irvine DV, Kloc A, Ren J, Li F, de Castro E, MarÃn L, Chang A, Goto D, Cande WZ, Antequera F, Arcangioli B, Martienssen R |
| Citation(s) |
22002604 |
| |
| Submission date |
Jul 28, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Mikel Zaratiegui |
| E-mail(s) |
zaratieg@cshl.edu
|
| Phone |
5163678836
|
| Fax |
5163678369
|
| Organization name |
Cold Spring Harbor Laboratory
|
| Department |
Delbruck Bldg.
|
| Lab |
Martienssen
|
| Street address |
1 Bungtown Rd
|
| City |
Cold Spring Harbor |
| State/province |
NY |
| ZIP/Postal code |
11724 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE30837 |
RNAi promotes heterochromatic silencing through replication-coupled release of RNA pol II (superseries) |
|
| Relations |
| SRA |
SRP007590 |
| BioProject |
PRJNA154613 |
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