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| Status |
Public on Jan 23, 2012 |
| Title |
Expression data in an Arabidopsis cytokinin-deficient mutant, ipt1,3,5,7 and its wild type plant (Col-0) under high salinity and control conditions. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
To understand the role of cytokinins (CKs) in salt stress response, we have employed transcriptional profiling of the CK-deficient mutant, ipt1,3,5,7 and wild type plant, Col-0 under high salinity and control conditions to identify genes differentially expressed in ipt1,3,5,7 under salt stress and control conditions. Agilent's Whole Arabidopsis Gene Expression Microarray (G2519F, V4, 4x44K) was used.
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| Overall design |
10-d-old plants grown on GM medium (22ºC, 16-h-light/8-h-dark) were transfered onto 1/2 MS agar plates without sucrose containing either 0 or 200 mM NaCl. Independent biological samples, 10 plants/each biological replicate, were collected after 24 hours. Total RNA was prepared and used for the microarray hybridization. Three replicative hybridization experiments were carried out for each independent biological sample.
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| Contributor(s) |
Nishiyama R, Le DT, Watanabe Y, Tanaka M, Morosawa T, Seki M, Yamaguchi-Shinozaki K, Shinozaki K, Tran LS |
| Citation(s) |
22355415 |
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| Submission date |
Sep 13, 2011 |
| Last update date |
Apr 22, 2015 |
| Contact name |
Rie Nishiyama |
| Organization name |
RIKEN
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| Department |
Plant Science Center
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| Lab |
Signaling Pathway Research Unit
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| Street address |
Suehiro-cho 1-7-22, Tsurumi
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| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL12621 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Probe Name version) |
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| Samples (12)
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| GSM795503 |
Col-0, Untreated plants, biological rep1 |
| GSM795504 |
Col-0, Untreated plants, biological rep2 |
| GSM795505 |
Col-0, Untreated plants, biological rep3 |
| GSM795506 |
ipt1357, Untreated plants, biological rep1 |
| GSM795507 |
ipt1357, Untreated plants, biological rep2 |
| GSM795508 |
ipt1357, Untreated plants, biological rep3 |
| GSM795509 |
Col-0, Salt-treated plants, biological rep1 |
| GSM795510 |
Col-0, Salt-treated plants, biological rep2 |
| GSM795511 |
Col-0, Salt-treated plants, biological rep3 |
| GSM795512 |
ipt1357, Salt-treated plants, biological rep1 |
| GSM795513 |
ipt1357, Salt-treated plants, biological rep2 |
| GSM795514 |
ipt1357, Salt-treated plants, biological rep3 |
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| Relations |
| BioProject |
PRJNA147575 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32087_RAW.tar |
91.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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