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Series GSE35211 Query DataSets for GSE35211
Status Public on Sep 01, 2016
Title HIP1 mediates prostate carcinogenesis and progression through STAT3 signalling
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Overexpression of HIP1 has been associated with fibroblast transformation, increasing grades of prostate cancer (CaP), and biochemical relapse. Here we demonstrate cellular transformation and phenotypic effects of HIP1 overexpression in a benign prostate epithelial cell line to be dependent on STAT3 signalling. In vivo xenografts confirmed the cellular transformation phenotype and revealed serum GDF15 to be a marker of CaP in our model. STAT3 signalling was in part, dependent on HIP1 expression, in a highly invasive, metastatic and androgen receptor (AR) negative DU145 cell line, Immunohistochemistry of a large prostate tissue microarray (TMA) revealed increased HIP1 and reciprocal GDF15 expression to adversely affect outcome warranting further studies to assess HIP1 and GDF15 as biomarkers for detection and prognostication of CaP.
 
Overall design Gene expression of PNT1a cells stably overexpressing HIP1 protein and LNCaP cells transiently overexpressing HIP1 protein were compared with their respective empty vector control cell lines using the illumina expression array platform. There were six biological replicates for each of the four different groups.
 
Contributor(s) Rao R, Mills I, Neal D, Russell R
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Submission date Jan 19, 2012
Last update date Aug 16, 2018
Contact name Roslin Russell
E-mail(s) roslin.russell@cruk.cam.ac.uk
Phone 01223 769770
Organization name Cambridge Research Institute, Cancer Research UK
Street address Robinson Way
City Cambridge
ZIP/Postal code CB2 0RE
Country United Kingdom
 
Platforms (1)
GPL6947 Illumina HumanHT-12 V3.0 expression beadchip
Samples (24)
GSM863727 PNT1a empty vector control-replicate 1
GSM863728 PNT1a empty vector control-replicate 2
GSM863729 PNT1a empty vector control-replicate 3
Relations
BioProject PRJNA150877

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE35211_RAW.tar 6.2 Mb (http)(custom) TAR
GSE35211_non-normalized.txt.gz 6.8 Mb (ftp)(http) TXT
Processed data included within Sample table
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