|
| Status |
Public on Sep 01, 2016 |
| Title |
LNCaP transiently overexpressing HIP1- replicate 4 |
| Sample type |
RNA |
| |
|
| Source name |
LNCaP cell line transiently overexpressing HIP1- Day 4 post transfection
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: LNCaP prostate cancer cell line
|
| Growth protocol |
PNT1a and LNCaP cells were cultured in RPMI supplemented with 10% FBS. PNT1a stable lines were maintained in 0.8 mg/ml G418 supplemented Media.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 4
|
| Data processing |
The bead-level data were preprocessed using BASH (Cairns et al. (2008) Bioinformatics 24(24):2921-2), a function from the beadarray package (Dunning et al (2007) Bioinformatics 23(16):2183-4) in Bioconductor, and also log base 2 transformed and quantile normalised using the beadarray package.
|
| |
|
| Submission date |
Jan 19, 2012 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Roslin Russell |
| E-mail(s) |
roslin.russell@cruk.cam.ac.uk
|
| Phone |
01223 769770
|
| Organization name |
Cambridge Research Institute, Cancer Research UK
|
| Street address |
Robinson Way
|
| City |
Cambridge |
| ZIP/Postal code |
CB2 0RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE35211 |
HIP1 mediates prostate carcinogenesis and progression through STAT3 signalling |
|
