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Series GSE36907 Query DataSets for GSE36907
Status Public on Sep 27, 2012
Title Cellular Origin and Pathophysiology of Chronic Lymphocytic Leukemia
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The cellular origin of chronic lymphocytic leukemia (CLL) is debated. Transcriptome analysis of CLL and normal peripheral blood and splenic B cell subsets displayed highest similarity of CLL to mature CD5+ B cells. We identified a distinct CD5+CD27+ post-germinal center B cell subset, and revealed that immunoglobulin V gene mutated CLL are more similar to mutated CD5+ B cells, whereas unmutated CLL are more related to unmutated CD5+ B cells. Stereotyped immunoglobulin V gene rearrangements were significantly enriched among CD5+ B cells, providing further genetic evidence for a derivation of CLL from CD5+ B cells. Moreover, we identified deregulated expression patterns providing novel insights into the pathophysiology of CLL, including downregulation of EBF1 and KLF family members.
Transcriptome profiling of CLL and healthy human blood and splenic mature B cell subsets. Identification of deregulated transcription patterns with implications on CLL pathobiology.
 
Overall design Human mature B cell subsets and CLL with mutated (mCLL) and unmutated V gene status (uCLL) were purified from peripheral blood and spleen. Samples of 5 to 7 donors each were collected and processed in three batches in a two rounded in vitro transcription protocol. Retrieved data were batch corrected and subjected to analysis.
Human mature CD5+ B cell subsets and CLL with mutated (mCLL) and unmutated V gene status (uCLL) were purified from peripheral blood.
 
Contributor(s) Seifert M, Kueppers R
Citation(s) 23091163
Submission date Mar 28, 2012
Last update date Mar 25, 2019
Contact name Marc JS Seifert
E-mail(s) marc.seifert@uni-due.de
Organization name University Hospital Essen
Department IFZ
Lab AGII
Street address Virchowstraße 173
City Essen
State/province NRW
ZIP/Postal code 45122
Country Germany
 
Platforms (2)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (65)
GSM905504 mature CD5+ PB B cells CD5_1
GSM905505 mature CD5+ PB B cells CD5_2
GSM905506 mature CD5+ PB B cells CD5_3
Relations
BioProject PRJNA157031

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Supplementary file Size Download File type/resource
GSE36907_RAW.tar 278.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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