|
| Status |
Public on Sep 27, 2012 |
| Title |
CLL with unmutated IgV gene umCLL_4 |
| Sample type |
RNA |
| |
|
| Source name |
human peripheral blood
|
| Organism |
Homo sapiens |
| Characteristics |
tumor stage: tumor
cell type: CLL cell with unmutated IgV gene
|
| Growth protocol |
cell sorting, see sample title
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10,000 cell samples sorted into Trizol lysis buffer, hydrous phase mixed with equal volume of 70% EtOH and loaded onto Qiagen RNeasy micro colum. Further processing according to manufacturer's instruction.
|
| Label |
biotin
|
| Label protocol |
Samples were processed with WT Ovation PICO RNA Amplification System, the WT Ovation Exon module and fragmented and labeled with the Encore Biotin module according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
cRNA was hybridized to GPL6244 Arrays with the Affymetrix GeneChip Hybridization, Wash and Stain kit, according to manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 7G and GeneChip Command Console 3.2 software.
|
| Description |
CD43 positive
|
| Data processing |
Data were normalized according to vsn and imported into Gene Spring GX 11.5.1 for further analysis.
|
| |
|
| Submission date |
Mar 28, 2012 |
| Last update date |
Sep 27, 2012 |
| Contact name |
Marc JS Seifert |
| E-mail(s) |
marc.seifert@uni-due.de
|
| Organization name |
University Hospital Essen
|
| Department |
IFZ
|
| Lab |
AGII
|
| Street address |
Virchowstraße 173
|
| City |
Essen |
| State/province |
NRW |
| ZIP/Postal code |
45122 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE36907 |
Cellular Origin and Pathophysiology of Chronic Lymphocytic Leukemia |
|
