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| Status |
Public on May 12, 2006 |
| Title |
Microarray analysis of human spermatogenic dysfunction |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We investigated testicular biopsies from 28 men which presented with full spermatogenesis (Johnsen Score 10, 12 samples), arrest at the spermatid stage (Johnsen Score 8, 6 samples), arrest at spermatocyte stage (Johnsen Score 5, 5 samples) and Sertoli-cell-only syndrom (Johnsen Score 2, 5 samples). Testicular biopsies were obtained by the "sandwich" method and conserved in RNALater (Ambion) during surgery.
Keywords:
spermatogenesis
germ cell differentiation
Keywords: disease state analysis
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| Overall design |
Total RNA was extracted from the biopsies by desintegration in RNAPure (Biozym) and post-cleanup with RNEasy columns (Qiagen). 2 ug of total RNA were labeled with the Amersham Codelink Expression Kit according to the manufacturers protocol, obtaining 40-80 ug of Biotin-labeled cRNA. 10 ug of fragmented cRNA were hybridized for 16h to Human 20K Codelink microarrays (Amersham), washed and stained with Cy5-Streptavidin according to manufacturers protocols and scanned with an Affymetrix 428 Array Scanner at gain 70. Spots were quantified by Codelink Expression software v4.0.
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| |
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| Contributor(s) |
Feig C, Kirchhoff C, Ivell R, Naether O, Schulze W, Spiess A |
| Citation(s) |
17114209 |
| |
| Submission date |
May 09, 2006 |
| Last update date |
Sep 27, 2013 |
| Contact name |
Andrej-Nikolai Spiess |
| E-mail(s) |
a.spiess@uke.uni-hamburg.de
|
| Phone |
+49-40-42803-1585
|
| Fax |
+49-40-42803-1554
|
| URL |
http://www.dr-spiess.de
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| Organization name |
University Hospital Hamburg-Eppendorf
|
| Department |
Andrology
|
| Lab |
Molecular Andrology
|
| Street address |
Martinistr. 52
|
| City |
20246 Hamburg |
| State/province |
n/a |
| ZIP/Postal code |
n/a |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL2891 |
GE Healthcare/Amersham Biosciences CodeLink™ UniSet Human 20K I Bioarray |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA95743 |
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