CodeLink UniSet Human Bioarray content was based on the public UniGene and RefSeq databases, and sequences for design were derived from Incyte LifeSeq™ Gold and Foundation databases. Well-annotated mRNA or coding sequences were chosen to ensure usefulness for a large range of applications in basic research, biotechnology, and drug development. Each sequence was carefully screened to ensure high quality, specific probe design, and to reduce redundancy of gene targets. Every probe was functionally validated against multiple tissues to ensure best representation of the gene so that the results will be biologically relevant. Every bioarray is also functionally validated during production to further ensure quality performance.
CodeLink users can submit data to GEO by exporting tab-delimited text files from the CodeLink software. The "Feature_id" header will need to be renamed to "ID_REF", and the "Normalized_intensity" header to "VALUE" before uploading the exported tables.
In addition, users are encouraged to include additional raw data columns in their data uploads. These additional columns do not need to be renamed.
Expression profiles of colorectal cancer cell lines
Data table header descriptions
ID
Feature_id in CodeLink data export
LOGICAL_ROW
The logical row position of the spot.
LOGICAL_COL
The logical column position of the spot.
PROBE_NAME
The unique probe identifier. Each Probe Name is specific for a given oligonucleotide probe and is the primary identifier.
PROBE_TYPE
A classifier indicating the functional role of the oligonucleotide probe.
PUB_PROBE_TARGETS
A classifier describing the oligonucleotide probe to target relationship. SINGLE - the oligo uniquely targets a single transcript in the public domain. MULTIPLE - the oligo targets more than one transcript in the public domain. DUPLICATE - more than one oligo exists on the bioarray that target the same transcript.
SPOT_ID
An identifier for oligonucleotide probes that do not have a corresponding public reference.
GB_ACC
The NCBI GenBank accession number assigned to the target sequence.
GI
The NCBI GI number assigned to the target sequence.
GB_LIST
A comma delimited list of NCBI GenBank accession numbers for probes having more than one target transcript.
GI_LIST
A comma delimited list of NCBI GI numbers for probes having more than one target transcript.
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