|
| Status |
Public on Jun 28, 2006 |
| Title |
Human decidual NK cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray analysis was performed on highly purified human CD56brightCD16- decidual NK cells pooled from healthy 1st trimester elective terminations. Experiment was performed to find out about genes expressed in this subset.
Keywords: Genes transcribed in cells studied
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| |
|
| Overall design |
Total cellular RNA from dNK subset was pooled and subjected to clean up protocol with RNeasy mini kits (Qiagen), according to the manufacturer’s specifications. First and second strand cDNA was prepared from RNA template, and the cDNA was subjected to in vitro transcription in the presence of biotinylated nucleoside triphosphates. The biotinylated cRNA was fragmented to uniform sizes (~100nt). CodeLinkTM Uniset Human 20K I Bioarrays (Amersham Biosceinces) were hybridized with each prepared cRNA target in duplicates and stained with Cy5-streptavidin and subsequently washed according to manufacturer’s instructions, and then scanned on an Axon GenePix 4000B scanner.
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| |
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| Citation(s) |
16892062 |
| |
| Submission date |
Jun 27, 2006 |
| Last update date |
Sep 27, 2013 |
| Contact name |
Ofer Mandelboim |
| E-mail(s) |
oferman@md2.huji.ac.il
|
| Phone |
972-26757515
|
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
The Lautenberg Center for General and Tumor Immunology
|
| Street address |
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL2891 |
GE Healthcare/Amersham Biosciences CodeLink™ UniSet Human 20K I Bioarray |
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| Samples (2) |
| GSM116327 |
Human decidual CD56+CD3-CD16- cells |
| GSM116328 |
Decidual CD56+CD3-CD16- (Replicate B) |
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| Relations |
| BioProject |
PRJNA96583 |
