|
| Status |
Public on Dec 26, 2013 |
| Title |
Generation of Rdp1-independent primary siRNAs |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We tested whether we could increase the population of Rdp1-independent primary siRNAs in fission yeast by overexpressing the Dcr1 ribonuclease. We found that in addition to generation of centromeric siRNAs, which was expected, Dcr1 overexpression also resulted in generation of genome-wide primary siRNAs, mapping to many open reading frames.
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| |
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| Overall design |
sequencing of 20-30nt sRNAs from total RNA
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| |
|
| Contributor(s) |
Yu R, Moazed D |
| Citation(s) |
24374313 |
| |
| Submission date |
Nov 19, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Ruby Yu |
| Organization name |
Harvard University
|
| Department |
Cell Biology
|
| Lab |
Danesh Moazed
|
| Street address |
240 Longwood Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02139 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13988 |
Illumina HiSeq 2000 (Schizosaccharomyces pombe) |
|
| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE52535 |
Determinants of heterochromatic siRNA biogenesis and function |
|
| Relations |
| BioProject |
PRJNA229166 |
| SRA |
SRP033132 |
