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Series GSE55924 Query DataSets for GSE55924
Status Public on Dec 02, 2014
Title Regulation of skeletal muscle energy/nutrient-sensing pathways during metabolic adaptation to fasting in healthy humans
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The aim of the study was to investigate how short-term fasting affects whole-body energy homeostasis and skeletal muscle energy/nutrient-sensing pathways and transcriptome in humans. For this purpose, twelve young healthy men were studied during a 24-hour fast. Skeletal muscle biopsies were collected and analyzed at baseline and after 4, 10 and 24h of fasting. As expected, fasting induced a time-dependent decrease in plasma insulin and leptin levels, whereas levels of ketone bodies and free fatty acids increased. This was associated with a metabolic shift from glucose towards lipid oxidation. Transcriptome profiling identified genes that were significantly regulated by fasting in skeletal muscle at both early and late time-points. Collectively, our study provides a comprehensive map of the main energy/nutrient-sensing pathways and transcriptomic changes during short-term adaptation to fasting in human skeletal muscle
 
Overall design Total RNA obtained from skeletal muscle biopsies collected at 1.5, 4 10 and 24h post-meal in healthy men subjected to a 24h-fast
 
Contributor(s) Guigas B, 't Hoen P
Citation(s) 25249505
Submission date Mar 14, 2014
Last update date Aug 13, 2018
Contact name Bruno Guigas
E-mail(s) b.g.a.guigas@lumc.nl
Organization name Leiden University Medical Center
Department Parasitology
Street address P.O.Box 9600, Postzone L40-Q
City Leiden
ZIP/Postal code 2300RC
Country Netherlands
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (48)
GSM1348553 Subject 1 - 1,5h post-meal
GSM1348554 Subject 1 - 4h post-meal
GSM1348555 Subject 1 - 10h post-meal
Relations
BioProject PRJNA241325

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55924_RAW.tar 26.2 Mb (http)(custom) TAR
GSE55924_non-normalized.txt.gz 16.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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