|
| Status |
Public on Dec 02, 2014 |
| Title |
Subject 6 - 24h post-meal |
| Sample type |
RNA |
| |
|
| Source name |
Human skeletal muscle
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Skeletal muscle (musculus vastus lateralis)
gender: Male
post-meal time: 24h
individual: Subject 6
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from skeletal muscle biopsies (~25-30 mg) using the phenol-chloroform extraction method (Tripure RNA Isolation reagent, Roche, Germany), treated with the TurboDNAse kit according to manufacturer protocol (TurboDNAse, Life Technologies, The Netherlands) and quantified by NanoDrop (ND1000, Thermo Scientific, The Netherlands). The quality and integrity of RNA was tested using 2100 Bioanalyzer (Agilent Technologies, Waldbronn, Germany) and all the samples had a RIN score >8.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Illumina TotalPrep-96 RNA Amplification kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol using Illumina’s Genomestudio v.2011.1
|
| Data processing |
The data were normalised using quantile normalisation with the lumi package version 2.4.0 in R
|
| |
|
| Submission date |
Mar 14, 2014 |
| Last update date |
Dec 02, 2014 |
| Contact name |
Bruno Guigas |
| E-mail(s) |
b.g.a.guigas@lumc.nl
|
| Organization name |
Leiden University Medical Center
|
| Department |
Parasitology
|
| Street address |
P.O.Box 9600, Postzone L40-Q
|
| City |
Leiden |
| ZIP/Postal code |
2300RC |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE55924 |
Regulation of skeletal muscle energy/nutrient-sensing pathways during metabolic adaptation to fasting in healthy humans |
|
