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| Status |
Public on Dec 01, 2014 |
| Title |
Ribosome Profiling in P. falciparum asexual blood stages |
| Organism |
Plasmodium falciparum |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The description of the transcriptome and proteome datasets of Plasmodium falciparum, the deadliest strain of human malaria, has been a tremendous resource for the understanding of the molecular physiology of this parasite. However, the underlying translational control that links global measurements of steady-state mRNA with protein levels is not well understood. Our work bridges this disconnect by measuring translation on a whole genome scale using ribosome profiling, providing the first measurements of new protein synthesis through the asexual blood phase developmental cycle.
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| Overall design |
Cultures corresponding to ring, early trophozoite, late trophozoite, schizont stage, merozoite were harvested for both mRNA and polysome isolation using cycloheximide to freeze ribosomes in place.
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| Contributor(s) |
Caro F, Ahyong V, Betegon M, DeRisi JL |
| Citation(s) |
25493618 |
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| Submission date |
Jun 11, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Joseph DeRisi |
| Organization name |
UCSF
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| Department |
Biochemistry
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| Lab |
DeRisi
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| Street address |
1700 4th Street
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94134 |
| Country |
USA |
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| Platforms (1) |
| GPL16607 |
Illumina HiSeq 2000 (Plasmodium falciparum) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA252511 |
| SRA |
SRP043116 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58402_RAW.tar |
133.1 Mb |
(http)(custom) |
TAR (of BED, TXT, WIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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