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Status |
Public on Dec 30, 2014 |
Title |
A global transcriptome analysis of keratinocytes upon overexpression of microRNA-132 |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
MiR-132 is one of the most upregulated miRNAs in keratinocytes of human skin wounds during the inflammatory phase of healing; however its biological role during skin wound healing has not been studied. To study the genes regulated by miR-132, we transfected miR-132 mimics (pre-miR-132) into primary human keratinocytes to overexpress miR-132. We performed a global transcriptome analysis of keratinocytes upon overexpression of miR-132 using Affymetrix arrays.
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Overall design |
Expression profiling of primary human keratinocytes transfected with 10nM miR-132 mimics (pre-miR-132) or control oligos (pre-miR-Ctrl) for 48 hours (biological triplicates in each group) was performed using Affymetrix GeneTitan system.
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Contributor(s) |
Landén NX |
Citation(s) |
26121747 |
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Submission date |
Sep 04, 2014 |
Last update date |
Jul 29, 2019 |
Contact name |
Ning Xu |
Organization name |
Karolinska Institutet
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Street address |
CMM L8:02
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City |
Stockholm |
ZIP/Postal code |
17176 |
Country |
Sweden |
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Platforms (1) |
GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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Samples (6)
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Relations |
BioProject |
PRJNA260313 |