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| Status |
Public on Dec 30, 2014 |
| Title |
A global transcriptome analysis of keratinocytes upon overexpression of microRNA-132 |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
MiR-132 is one of the most upregulated miRNAs in keratinocytes of human skin wounds during the inflammatory phase of healing; however its biological role during skin wound healing has not been studied.
To study the genes regulated by miR-132, we transfected miR-132 mimics (pre-miR-132) into primary human keratinocytes to overexpress miR-132.
We performed a global transcriptome analysis of keratinocytes upon overexpression of miR-132 using Affymetrix arrays.
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| Overall design |
Expression profiling of primary human keratinocytes transfected with 10nM miR-132 mimics (pre-miR-132) or control oligos (pre-miR-Ctrl) for 48 hours (biological triplicates in each group) was performed using Affymetrix GeneTitan system.
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| Contributor(s) |
Landén NX |
| Citation(s) |
26121747 |
| |
| Submission date |
Sep 04, 2014 |
| Last update date |
Jul 29, 2019 |
| Contact name |
Ning Xu |
| Organization name |
Karolinska Institutet
|
| Street address |
CMM L8:02
|
| City |
Stockholm |
| ZIP/Postal code |
17176 |
| Country |
Sweden |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA260313 |
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