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Status |
Public on Dec 30, 2014 |
Title |
keratinocytes_pre-miR-132_rep1 |
Sample type |
RNA |
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Source name |
keratinocytes_pre-miR-132
|
Organism |
Homo sapiens |
Characteristics |
tissue: skin cell type: primary human keratinocytes transfected with: 10nM miR-132 mimics (pre-miR-132)
|
Treatment protocol |
The third passage keratinocytes at 30-50% confluence were transfected with 10 nM miR-132 mimics or control oligos (Ambion) using Lipofectamine 2000 (Invitrogen).
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Growth protocol |
Human adult epidermal keratinocytes were purchased from Cascade Biologics and cultured in EpiLife® serum-free keratinocyte growth medium including Human Keratinocyte Growth Supplement at a final Ca2+ concentration of 0.06mM (Cascade Biologics) at 37°C in 5% CO2.
|
Extracted molecule |
total RNA |
Extraction protocol |
48 hours after transfection, the total RNA was extracted with miRNeasy Mini kit (Qiagen) according to the manufacturer's instructions.
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Label |
biotin
|
Label protocol |
Total RNA was labeled using the Whole Transcript (WT) Sense Target Labeling Assay according to the manufacturer’s (Affymetrix) instructions.
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|
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Hybridization protocol |
The samples were hybridized to a Human Gene 1.1 ST Array Plate according to standard Affymetrix GeneTitan protocol.
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Scan protocol |
The array plate was scanned using the Affymetrix GeneTitan Scanner.
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Description |
Gene expression data from keratinocytes with miR-132 overexpression. pre-miR-132a
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Data processing |
Generated CEL files were analyzed using Affymetrix Expression Console v. 1.1 and the RMA method
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Submission date |
Sep 04, 2014 |
Last update date |
Dec 30, 2014 |
Contact name |
Ning Xu |
Organization name |
Karolinska Institutet
|
Street address |
CMM L8:02
|
City |
Stockholm |
ZIP/Postal code |
17176 |
Country |
Sweden |
|
|
Platform ID |
GPL6244 |
Series (1) |
GSE61139 |
A global transcriptome analysis of keratinocytes upon overexpression of microRNA-132 |
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