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| Status |
Public on Jul 01, 2015 |
| Title |
Expression data of leaves from transgenic barley expressing wheat Lr34 gene |
| Organism |
Hordeum vulgare |
| Experiment type |
Expression profiling by array
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| Summary |
The wheat gene Lr34 (Yr18/Pm38/Sr57/Ltn1) encodes a putative ABCG-type of transporter and is a unique source of disease resistance providing durable and partial resistance against multiple fungal pathogens. Lr34 has been found to be functional as a transgene in barley.
We used microarrays to decipher the changes in global gene expression goverened by Lr34 expression in barley.
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| Overall design |
Leaves from two indipendent lines of transgenic barley plants were selected at seedling (Two weeks old plants) and mature (six weeks old plants) stages of development for RNA extraction and hybridization on Affymetrix microarrays. We used three biological replicates for expression profiles. Wild type plants served as controls.
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| Contributor(s) |
Beat K, Harsh C |
| Citation(s) |
26315512 |
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| Submission date |
Dec 16, 2014 |
| Last update date |
Oct 07, 2015 |
| Contact name |
Harsh Chauhan |
| Organization name |
Indian Institute of Technology Roorkee
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| Department |
Department of Biotechnology
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| Lab |
G10
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| Street address |
Old Biotechnology Building
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| City |
Roorkee |
| State/province |
Uttaranchal |
| ZIP/Postal code |
247667 |
| Country |
India |
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| Platforms (1) |
| GPL1340 |
[Barley1] Affymetrix Barley Genome Array |
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| Samples (18)
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| GSM1566644 |
Transgenic line BG8 seedling leaf, biological rep1 |
| GSM1566645 |
Transgenic line BG8 seedling leaf, biological rep2 |
| GSM1566646 |
Transgenic line BG8 seedling leaf, biological rep3 |
| GSM1566647 |
Transgenic line BG9 seedling leaf, biological rep1 |
| GSM1566648 |
Transgenic line BG9 seedling leaf, biological rep2 |
| GSM1566649 |
Transgenic line BG9 seedling leaf, biological rep3 |
| GSM1566650 |
wild type mature, biological rep1 |
| GSM1566651 |
wild type mature, biological rep2 |
| GSM1566652 |
wild type mature, biological rep3 |
| GSM1566653 |
Transgenic line BG8 mature leaf, biological rep1 |
| GSM1566654 |
Transgenic line BG8 mature leaf, biological rep2 |
| GSM1566655 |
Transgenic line BG8 mature leaf, biological rep3 |
| GSM1566656 |
Transgenic line BG9 mature leaf, biological rep1 |
| GSM1566657 |
Transgenic line BG9 mature leaf, biological rep2 |
| GSM1566658 |
Transgenic line BG9 mature leaf, biological rep3 |
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| Relations |
| BioProject |
PRJNA270487 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64228_RAW.tar |
38.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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