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| Status |
Public on Jul 01, 2015 |
| Title |
Microarray analysis to identify the differentially expressed genes induced by NROB |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Compared to wild type plants, overexpression of NROB results leaf early senescence, biomass and seeds yield decreased more than 70%. Microarray analysis the differential expression genes induced by NROB
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| Overall design |
Young leaves of DAG 30 were harvested from WT and 35S: NROB #2 plants and frozen immediately in liquid nitrogen. Total RNA was extracted using the RNeasy plant mini kit (Qiagen) and was checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100. Qualified and purified total RNA was amplified and labeled by Low Input Quick Amp Labeling Kit, Two-Color (Agilent technologies, US). Regulated genes were identified with a stringent significance threshold, namely a mean >2.0-fold change (transformants relative to WT control samples) and based on at least two replicates. Microarray analysis result was verified by real-time PCR
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| Contributor(s) |
Yang H, Liu J |
| Citation missing |
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| Submission date |
Jan 30, 2015 |
| Last update date |
Jul 02, 2015 |
| Contact name |
Hong li Yang |
| E-mail(s) |
yanghongli1983@163.com
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| Phone |
027-86712076
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Department |
Oil Crops Research Institute
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| Lab |
Key Laboratory of Biology and Genetic Improvement of Oil Crops
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| Street address |
xudong 2 road
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| City |
wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430062 |
| Country |
China |
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| Platforms (1) |
| GPL12621 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Probe Name version) |
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| Samples (2) |
| GSM1598046 |
Control leaf vs transgenic NROB leaf Replicate 1 |
| GSM1598047 |
Control leaf vs transgenic NROB leaf Replicate 2 |
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| Relations |
| BioProject |
PRJNA274045 |
