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| Status |
Public on Feb 03, 2015 |
| Title |
Real Time PCR analysis of T helper cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by RT-PCR
|
| Summary |
Murine lymphocytes T helper cells were obtained from spleen of C57Bl/6 mice and miR-155 deficient mice strains that have been immunised with MOG peptide 35-55 in complete Freunds adiuvant 12 days prior.
Subsequently cells were stimulated for 72 hours with MOG peptide 35-55 or left unstimulated followed by T helper cell sorting using magnetic beads and RNA isolation.
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| Overall design |
qPCR gene expression profiling. Cells from three individual mice of the same genotype were pooled and treated separately as indicated in the summary. Equal amount total RNA from each mice was pooled prior to gene expression analysis.
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| Contributor(s) |
Mycko MP, Cichalewska M |
| Citation(s) |
26674874 |
| |
| Submission date |
Jan 30, 2015 |
| Last update date |
Jan 05, 2016 |
| Contact name |
Marcin P Mycko |
| E-mail(s) |
mm@afazja.am.lodz.pl
|
| Phone |
4842 67577603
|
| Organization name |
Medical University of Lodz
|
| Department |
Department of Neurology
|
| Lab |
Laboratory of Neuroimmunology
|
| Street address |
Kopcinskiego 22
|
| City |
Lodz |
| ZIP/Postal code |
90-153 |
| Country |
Poland |
| |
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| Platforms (1) |
| GPL19725 |
LifeTechnologies TaqMan assays card |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA274190 |
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