|
| Status |
Public on Feb 03, 2015 |
| Title |
B6_MOG35-55 |
| Sample type |
RNA |
| |
|
| Source name |
T helper cells from murine splenocytes
|
| Organism |
Mus musculus |
| Characteristics |
cell type: T helper cells
activation: MOG peptide 35-55 20 ug/ml
time of stimulation: 72 hrs
strain: C57Bl/6
|
| Treatment protocol |
20 ug/ml of MOG peptide 35-55 or untreated
|
| Growth protocol |
72 hrs in 37C in IMDM/10% FCS medium 1x10e6 cells/ml
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA isolation with miRVana
|
| Label |
TaqMan probes (FAM)
|
| Label protocol |
PCR assays were performed using the TaqMan probes (Life Technologies).
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Test
|
| Data processing |
endogenous control Hprt1 oraz 18-s; Target gene signals normalized to housekeeping genes; 2^-deltaCt, where deltaCt = (Ct_Target − Ct_HKG)];
DataAssist (Life Technologies) software performed all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data; Matrix normalized worksheet reports normalized signal (against housekeeping genes); Fold Change worksheet reports test/control ratios.
|
| |
|
| Submission date |
Jan 30, 2015 |
| Last update date |
Feb 03, 2015 |
| Contact name |
Marcin P Mycko |
| E-mail(s) |
mm@afazja.am.lodz.pl
|
| Phone |
4842 67577603
|
| Organization name |
Medical University of Lodz
|
| Department |
Department of Neurology
|
| Lab |
Laboratory of Neuroimmunology
|
| Street address |
Kopcinskiego 22
|
| City |
Lodz |
| ZIP/Postal code |
90-153 |
| Country |
Poland |
| |
|
| Platform ID |
GPL19725 |
| Series (1) |
| GSE65488 |
Real Time PCR analysis of T helper cells |
|
