|
| Status |
Public on Sep 01, 2016 |
| Title |
DNA methylation profiling of rhabdomyosarcoma tumor samples |
| Organism |
Homo sapiens |
| Experiment type |
Methylation profiling by genome tiling array
|
| Summary |
In order to identify the specific DNA methylation pattern of different rhabdomyosarcoma (RMS) samples we performed a genome-wide study using Human DNA methylation platform (Agilent).
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| |
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| Overall design |
DNA methylation profiling was carried out in 15 RMS tumor samples using the Human DNA methylation microarray (Agilent) consisting of about 244,000 (60-mer) probes design to interrogate about 27,000 known CpG islands. Enriched-methylated dsDNA for each sample was labeled with Cy5 dye and the control genomic DNA for each sample was labeled with Cy3 dye using Agilent Genomic DNA labeling kit PLUS (Agilent).
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| |
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| Contributor(s) |
Tombolan L, Celegato B, Millino C, Pacchioni B, Martini P, Romualdi C, Zin A, Poli E, Rosolen A, Lanfranchi G |
| Citation(s) |
27842508 |
| |
| Submission date |
Mar 24, 2015 |
| Last update date |
Dec 01, 2016 |
| Contact name |
Gerolamo Lanfranchi |
| E-mail(s) |
stefano.cagnin@unipd.it
|
| Phone |
+39-0498276219
|
| Organization name |
University of Padova
|
| Department |
CRIBI - Biotechnology Center and Biology Department
|
| Lab |
Functional Genomics Lab
|
| Street address |
Via U. Bassi, 58/B
|
| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL19930 |
Agilent-023795 Human DNA Methylation Microarray 244k (Probe Name Version) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA279257 |
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