Acquired imatinib resistance in chronic myelogenous leukemia (CML) can be the consequence of mutations in the kinase domain of BCR-ABL or increased protein levels. However, as in other malignancies, acquired resistance to cytostatic drugs is a common reason for treatment failure or disease progression. As a model for drug resistance, we developed a CML cell line resistant to cyclophosphamide (CP). Using oligonucleotide arrays, we examined changes in global gene expression. Selected genes were also examined by real-time PCR and flow cytometry. Neither the parent nor the resistant lines had mutations in their ATP binding domain. Filtering genes with a low-base line expression, a total of 239 genes showed significant changes (162 up- and 77 down-regulated) in the resistant clone. Most of the up-regulated genes were associated with metabolism, signal transduction, or encoded enzymes. The gene for aldehyde dehydrogenase 1 was over-expressed more than 2000 fold in the resistant clone. BCR-ABL was expressed in both cell lines to a comparable extent. When exposed to the tyrosine kinase inhibitors imatinib and nilotinib, both lines were sensitive. In conclusion, we found multiple genetic changes in a CML cell line resistant to CP related to metabolism, signal transduction or apoptosis. Despite these changes, the resistant cells retained sensitivity to tyrosine kinase inhibitors. Keywords: Cell type comparison
Overall design
A CML cell line and a CP resistant subline were compared by gene array. In addition other markers, the expression of BCR-ABL and the sensitivity to tyrosine kinase inhibitors were tested.
Table 1: Stringent List of 251 genes (Filters applied:1-Samples were normalized in GeneChip Operating Software; 2-Absolute pivot data was uploaded into GeneSifter where comparisons and t-test were performed; 3-Samples were filtered on t-test p-value ,>.05 filtered out; 4-Samples were filtered on Detection Calls, 100% Absent filtered out; 5-Samples were filtered on Signal ,<500 in both Parental and Resistant Groups filtered out; 6-Samples were filtered on Signal Log Ratio of 2-fold or greater in all comparisons; 7-Samples were filtered on Change Calls, must be at least 80% concordance of calls) header descriptions
Probe Set ID
Sensitive Mean
Sensitive SEM
Resistant Mean
Resistant SEM
Ratio
Direction
p-value
adj p-value
Gene Name
Gene ID
S Signal
S Detection
S Detection p-value
P1 Signal
P1 Detection
P1 Detection p-value
P2 Signal
P2 Detection
P2 Detection p-value
P3 Signal
P3 Detection
P3 Detection p-value
K Signal
K Detection
K Detection p-value
R1 Signal
R1 Detection
R1 Detection p-value
R2 Signal
R2 Detection
R2 Detection p-value
R3 Signal
R3 Detection
R3 Detection p-value
R4 Signal
R4 Detection
R4 Detection p-value
delta Signal
delta Detection
delta Detection p-value
R1 vs S Signal Log Ratio
R1 vs S Change
R1 vs S Change p-value
R2 vs P1 Signal Log Ratio
R2 vs P1 Change
R2 vs P1 Change p-value
R3 vs P2 Signal Log Ratio
R3 vs P2 Change
R3 vs P2 Change p-value
R4 vs P3 Signal Log Ratio
R4 vs P3 Change
R4 vs P3 Change p-value
delta vs K Signal Log Ratio
delta vs K Change
delta vs K Change p-value
Absent Count
Absent %
NC Count
NC %
Increase Count
Increase %
Decrease Count
Decrease %
Gene Symbol
Gene Title
P Avg Signal
R Avg Signal
P<500
R<500
P&R Sum
Binned R2 vs P1 Signal Log Ratio
Binned R1 vs S Signal Log Ratio
Binned R3 vs P2 Signal Log Ratio
Binned R4 vs P3 Signal Log Ratio
Binned delta vs K Signal Log Ratio
Data table
Probe Set ID
Sensitive Mean
Sensitive SEM
Resistant Mean
Resistant SEM
Ratio
Direction
p-value
adj p-value
Gene Name
Gene ID
S Signal
S Detection
S Detection p-value
P1 Signal
P1 Detection
P1 Detection p-value
P2 Signal
P2 Detection
P2 Detection p-value
P3 Signal
P3 Detection
P3 Detection p-value
K Signal
K Detection
K Detection p-value
R1 Signal
R1 Detection
R1 Detection p-value
R2 Signal
R2 Detection
R2 Detection p-value
R3 Signal
R3 Detection
R3 Detection p-value
R4 Signal
R4 Detection
R4 Detection p-value
delta Signal
delta Detection
delta Detection p-value
R1 vs S Signal Log Ratio
R1 vs S Change
R1 vs S Change p-value
R2 vs P1 Signal Log Ratio
R2 vs P1 Change
R2 vs P1 Change p-value
R3 vs P2 Signal Log Ratio
R3 vs P2 Change
R3 vs P2 Change p-value
R4 vs P3 Signal Log Ratio
R4 vs P3 Change
R4 vs P3 Change p-value
delta vs K Signal Log Ratio
delta vs K Change
delta vs K Change p-value
Absent Count
Absent %
NC Count
NC %
Increase Count
Increase %
Decrease Count
Decrease %
Gene Symbol
Gene Title
P Avg Signal
R Avg Signal
P<500
R<500
P&R Sum
Binned R2 vs P1 Signal Log Ratio
Binned R1 vs S Signal Log Ratio
Binned R3 vs P2 Signal Log Ratio
Binned R4 vs P3 Signal Log Ratio
Binned delta vs K Signal Log Ratio
37015_at
0.00642
0.001568
17.174999
0.932049
2675.23
Up
7.77E-08
1.37E-05
Human aldehyde dehydrogenase 1 mRNA
ALDH1A1
8.9
A
0.926238
19.9
A
0.96066
47.4
A
0.975289
23.9
A
0.993468
39.7
A
0.933168
64279
P
0.000219
67196.8
P
0.000219
64840.1
P
0.000219
86860.7
P
0.000219
86580.4
P
0.000219
12.65
I
0
11.38
I
0
9.66
I
0
11.38
I
0
10.76
I
0
5
50
0
0
5
100
0
0
ALDH1A1
aldehyde dehydrogenase 1 family, member A1
27.96
73951.4
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
39665_at
0.014059
0.003604
1.629749
0.171023
115.92
Up
1.30E-05
0.000297286
Human glycine receptor beta subunit (GLRB) mRNA, complete cds
GLRB
44.8
A
0.882879
38
A
0.978134
35.5
A
0.996938
67.7
A
0.715253
120.3
A
0.52062
5054.4
P
0.000468
7539.5
P
0.000322
8128.6
P
0.000468
5393.3
P
0.000322
8866.8
P
0.000322
6.6
I
1.00E-06
7.56
I
1.00E-06
6.56
I
0
5.86
I
0
5.01
I
1.00E-06
5
50
0
0
5
100
0
0
GLRB
glycine receptor, beta
61.26
6996.52
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
32747_at
0.037825
0.021726
3.456661
0.083305
91.39
Up
1.78E-10
3.64E-07
Human RNA for mitochondrial aldehyde dehydrogenase I ALDH I (EC 1.2.1.3)
ALDH2
70.5
A
0.968664
92.5
A
0.795978
61.5
A
0.992457
535
A
0.67917
56.8
A
0.988553
14941.4
P
0.000805
13338.5
P
0.000805
14891.1
P
0.000959
14740.9
P
0.001602
16269.8
P
0.000673
7.37
I
6.00E-06
7.14
I
6.00E-06
7.6
I
5.00E-06
4.77
I
6.00E-06
8.22
I
5.00E-06
5
50
0
0
5
100
0
0
ALDH2
aldehyde dehydrogenase 2 family (mitochondrial)
163.26
14836.34
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
40480_s_at
0.012239
0.002991
0.923645
0.1014
75.47
Up
1.88E-05
0.000354046
Homo sapiens c-syn protooncogene mRNA, complete cds
FYN
66.5
A
0.964837
96.3
A
0.715253
30
A
0.994357
33.1
A
0.986921
39.4
A
0.993468
2959.3
P
0.000322
4052.3
P
0.000322
5490.2
P
0.000219
3719.6
P
0.000219
3567.6
P
0.000219
5.24
I
0
4.82
I
0
7.52
I
0
6.92
I
0
6.34
I
0
5
50
0
0
5
100
0
0
FYN
FYN oncogene related to SRC, FGR, YES
53.06
3957.8
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
1929_at
0.026794
0.0144
1.985909
0.167888
74.12
Up
2.73E-06
0.000113812
HSU83508 Human angiopoietin-1 mRNA, complete cds
ANGPT1
13.7
A
0.88284
163.9
A
0.378165
33.6
A
0.765443
336.3
A
0.175949
27.2
A
0.541184
6377.2
P
0.000266
10639
P
0.000266
8003.2
P
0.000266
8387
P
0.000265
9182.9
P
0.000219
7.48
I
0
5.42
I
1.00E-06
6
I
0
4.41
I
1.00E-06
6.72
I
0
5
50
0
0
5
100
0
0
ANGPT1
angiopoietin 1
114.94
8517.86
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
2039_s_at
0.021775
0.006639
1.259766
0.194134
57.85
Up
0.000215187
0.001803511
HUMCSYNA Homo sapiens c-syn protooncogene mRNA, complete cds
FYN
111.2
A
0.872355
32.6
A
0.975308
202.2
A
0.781055
62.6
A
0.933135
68.9
A
0.697453
3923.8
P
0.003585
4807.9
P
0.002617
8475.4
P
0.000805
5634.6
P
0.017001
4162.6
P
0.00418
5.26
I
3.00E-06
6.33
I
1.00E-06
5.5
I
1.00E-06
6.25
I
9.00E-06
5.72
I
3.00E-06
5
50
0
0
5
100
0
0
FYN
FYN oncogene related to SRC, FGR, YES
95.5
5400.86
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
38047_at
0.009074
0.001925
0.52256
0.085992
57.59
Up
0.000334477
0.002414237
Homo sapiens mRNA for RBP-MS
RBPMS
19.7
A
0.997776
26.1
A
0.993468
34.7
A
0.995825
64.1
A
0.990015
52.5
A
0.964837
2171.6
P
0.000805
3520.3
P
0.000322
1951.2
P
0.000468
1634.3
P
0.000388
1841.6
P
0.005643
6.65
I
1.00E-06
6.19
I
0
5.77
I
0
4.82
I
0
4.83
I
7.00E-06
5
50
0
0
5
100
0
0
RBPMS
RNA binding protein with multiple splicing
39.42
2223.8
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
39070_at
0.043884
0.020594
2.42416
0.286624
55.24
Up
3.40E-05
0.000469339
Human actin bundling protein (HSN) mRNA, complete cds
FSCN1
56.3
A
0.765481
42.4
A
0.602006
501
A
0.250724
62.6
A
0.561639
299.4
A
0.218983
13746.5
P
0.000219
11601.4
P
0.000266
7193.4
P
0.000218
9321.6
P
0.000322
9889.2
P
0.000266
7.53
I
0
6.85
I
2.00E-06
3.13
I
1.90E-05
7.05
I
0.00057
4.43
I
1.00E-06
5
50
0
0
5
100
0
0
FSCN1
fascin homolog 1, actin-bundling protein (Strongylocentrotus purpuratus)
192.34
10350.42
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
37605_at
0.02027
0.003473
1.062969
0.160156
52.44
Up
0.000186335
0.001628441
Human pro-alpha1 type II collagen (COL2A1) gene exons 1-54, complete cds
-
70.1
A
0.679198
129.2
A
0.660442
101.7
A
0.781017
43.4
A
0.861235
95.8
A
0.892699
6482.5
P
0.000266
4314.9
P
0.000468
2574.2
P
0.001602
3986.4
P
0.000219
5389.4
P
0.000468
6.01
I
0
5.15
I
1.50E-05
4.48
I
5.00E-06
6.09
I
4.00E-06
5.05
I
1.00E-06
5
50
0
0
5
100
0
0
COL2A1
collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital)
Homo sapiens mRNA for KIAA0920 protein, complete cds
PALM2-AKAP2
258.4
A
0.795978
45.5
A
0.933135
535.8
A
0.189648
163.1
A
0.824011
148.2
A
0.64131
6009.8
P
0.000219
9470.6
P
0.000219
9515.2
P
0.000266
11565.5
P
0.000219
17672.2
P
0.000219
4.58
I
1.00E-06
6.83
I
0
4.39
I
1.00E-06
6.34
I
0
6.13
I
0
5
50
0
0
5
100
0
0
AKAP2
A kinase (PRKA) anchor protein 2
230.2
10846.66
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
32434_at
0.022587
0.014304
1.077887
0.051769
47.72
Up
4.68E-08
1.08E-05
Homo sapiens mRNA for 80K-L protein, complete cds
MARCKS
18.3
A
0.997383
18.9
A
0.92617
341.7
A
0.458809
101.9
A
0.933135
13
A
0.939581
5068
P
0.000219
4877.5
P
0.000219
4111.7
P
0.001141
4449.1
P
0.000959
4563.6
P
0.000219
7.67
I
0
6.84
I
0
3.66
I
8.00E-06
5.96
I
3.00E-06
6.65
I
0
5
50
0
0
5
100
0
0
MARCKS
myristoylated alanine-rich protein kinase C substrate
98.76
4613.98
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
38323_at
0.018537
0.010966
0.877019
0.066621
47.31
Up
1.38E-06
6.87E-05
Homo sapiens BAC clone RG113D17 from 7p14-p15
-
21.6
A
0.986908
14.9
A
0.964861
271.8
A
0.781017
36.7
A
0.983
61.3
A
0.749276
3951.9
P
0.000219
4480.2
P
0.000219
3226.1
P
0.000266
3182.4
P
0.000673
3910.5
P
0.000388
7.21
I
0
7.7
I
1.00E-06
3.47
I
4.00E-06
5.78
I
0
5.6
I
0
5
50
0
0
5
100
0
0
CPVL
carboxypeptidase, vitellogenic-like
81.26
3750.22
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
39315_at
0.058059
0.017528
2.423565
0.116738
41.74
Up
4.01E-08
1.08E-05
Human mRNA for KIAA0003 gene, complete cds
ANGPT1
63.4
A
0.438361
407.7
A
0.150527
86
A
0.732572
291.6
A
0.339558
404.1
A
0.175989
10441
P
0.000266
9456.1
P
0.000219
9042.7
P
0.000266
10562.8
P
0.000219
12574.5
P
0.000219
6.94
I
0
4.03
I
0
6.49
I
1.00E-06
5.05
I
0
4.68
I
1.00E-06
5
50
0
0
5
100
0
0
ANGPT1
angiopoietin 1
250.56
10415.42
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
37641_at
0.031015
0.014759
1.220722
0.074195
39.36
Up
2.67E-07
2.31E-05
Human gene for hepatitis C-associated microtubular aggregate protein p44
-
22.5
A
0.945559
212.6
A
0.602006
22.1
A
0.956032
342.7
M
0.043968
65.5
A
0.88284
5880
P
0.000219
5358.5
P
0.000388
4118.6
P
0.000468
5481
P
0.000266
5317.5
P
0.000266
7.22
I
0
4.29
I
2.00E-06
6.2
I
1.00E-06
3.49
I
1.00E-06
5.67
I
0
4
40
0
0
5
100
0
0
IFI44
interferon-induced protein 44
133.08
5231.12
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
37021_at
0.137294
0.022621
5.097328
0.417092
37.13
Up
2.32E-06
0.000101058
Human mRNA for cathepsin H (EC 3.4.22.16)
CTSH
550
A
0.250724
313.2
A
0.458816
921
A
0.284747
688.8
A
0.218945
523.8
A
0.138765
24025.3
P
0.000322
22220.9
P
0.000219
14716.7
P
0.000266
24578.2
P
0.000266
23855.1
P
0.000219
5.05
I
1.00E-06
5.54
I
1.00E-06
3.54
I
1.00E-06
5.32
I
1.00E-06
5.04
I
0
5
50
0
0
5
100
0
0
CTSH
cathepsin H
599.36
21879.24
0
0
0
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
32889_at
0.037065
0.012943
1.359886
0.070809
36.69
Up
7.91E-08
1.37E-05
td18e08.x1 Homo sapiens cDNA, 3 end
RPIB9
227.3
A
0.581931
87.8
A
0.795978
350.7
A
0.561649
23.4
A
0.849513
124.8
A
0.520623
5019.8
P
0.000266
5866.8
P
0.000266
6835.5
P
0.000266
5822.1
P
0.000266
5609.3
P
0.000219
4.23
I
0
6.25
I
1.00E-06
3.78
I
1.00E-06
7.34
I
1.00E-06
5.35
I
0
5
50
0
0
5
100
0
0
RPIB9
Rap2-binding protein 9
162.8
5830.7
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
1837_at
0.011053
0.004062
0.396152
0.048516
35.84
Up
4.74E-05
0.000583594
Ras-Like Protein Tc21
-
27.4
A
0.849513
112.6
A
0.810313
15.3
A
0.975289
38.8
A
0.861235
43.7
A
0.892737
1630.3
P
0.000959
2187.2
P
0.000562
1797.3
P
0.000562
1010.4
P
0.000266
1827.6
P
0.001354
5.31
I
1.20E-05
3.57
I
1.00E-06
5.54
I
5.00E-06
5.16
I
1.00E-06
5.39
I
1.20E-05
5
50
0
0
5
100
0
0
---
---
47.56
1690.56
1
0
1
0.99 < x
0.99 < x
0.99 < x
0.99 < x
0.99 < x
Total number of rows: 251
Table truncated, full table size 125 Kbytes.
Table 2: Filtered List header descriptions
Probe Set ID
P Avg Signal
R Avg Signal
P<500
R<500
Sum of D2 & E2
Sensitive Mean
Sensitive SEM
Resistant Mean
Resistant SEM
Ratio
Direction
p-value
adj p-value
Gene Name
Gene ID
S Signal
S Detection
S Detection p-value
P1 Signal
P1 Detection
P1 Detection p-value
P2 Signal
P2 Detection
P2 Detection p-value
P3 Signal
P3 Detection
P3 Detection p-value
K Signal
K Detection
K Detection p-value
R1 Signal
R1 Detection
R1 Detection p-value
R2 Signal
R2 Detection
R2 Detection p-value
R3 Signal
R3 Detection
R3 Detection p-value
R4 Signal
R4 Detection
R4 Detection p-value
delta Signal
delta Detection
delta Detection p-value
R1 vs S Signal Log Ratio
R1 vs S Change
R1 vs S Change p-value
R2 vs P1 Signal Log Ratio
R2 vs P1 Change
R2 vs P1 Change p-value
R3 vs P2 Signal Log Ratio
R3 vs P2 Change
R3 vs P2 Change p-value
R4 vs P3 Signal Log Ratio
R4 vs P3 Change
R4 vs P3 Change p-value
delta vs K Signal Log Ratio
delta vs K Change
delta vs K Change p-value
Absent Count
Absent %
NC Count
NC %
Increase Count
Increase %
Decrease Count
Decrease %
Gene Symbol
Gene Title
Data table
Probe Set ID
P Avg Signal
R Avg Signal
P<500
R<500
Sum of D2 & E2
Sensitive Mean
Sensitive SEM
Resistant Mean
Resistant SEM
Ratio
Direction
p-value
adj p-value
Gene Name
Gene ID
S Signal
S Detection
S Detection p-value
P1 Signal
P1 Detection
P1 Detection p-value
P2 Signal
P2 Detection
P2 Detection p-value
P3 Signal
P3 Detection
P3 Detection p-value
K Signal
K Detection
K Detection p-value
R1 Signal
R1 Detection
R1 Detection p-value
R2 Signal
R2 Detection
R2 Detection p-value
R3 Signal
R3 Detection
R3 Detection p-value
R4 Signal
R4 Detection
R4 Detection p-value
delta Signal
delta Detection
delta Detection p-value
R1 vs S Signal Log Ratio
R1 vs S Change
R1 vs S Change p-value
R2 vs P1 Signal Log Ratio
R2 vs P1 Change
R2 vs P1 Change p-value
R3 vs P2 Signal Log Ratio
R3 vs P2 Change
R3 vs P2 Change p-value
R4 vs P3 Signal Log Ratio
R4 vs P3 Change
R4 vs P3 Change p-value
delta vs K Signal Log Ratio
delta vs K Change
delta vs K Change p-value
Absent Count
Absent %
NC Count
NC %
Increase Count
Increase %
Decrease Count
Decrease %
Gene Symbol
Gene Title
36367_at
573.96
31.72
0
1
1
0.131476
0.013732
0.007297
0.001661
18.02
Down
1.89E-05
0.000354046
Human clone 23599 mRNA sequence
PCDH11X
765.5
P
0.024711
424.1
A
0.175949
633.4
A
0.32083
606.3
A
0.127645
440.5
A
0.204022
15.2
A
0.978134
20.6
A
0.956032
29.5
A
0.926204
33.7
A
0.975308
59.6
A
0.951005
-5.1
D
0.999941
-3.64
D
0.99971
-4.12
NC
0.994506
-4.29
NC
0.996147
-2.29
D
0.999186
9
90
2
40
0
0
3
60
PCDH11X
protocadherin 11 X-linked
38172_at
119.84
624.62
1
0
1
0.027484
0.012121
0.145271
0.016594
5.29
Up
0.000438056
0.002917997
Homo sapiens DNA, chromosome 21q22.2, PAC clone 25P16 complete sequence, encoding carbonyl reductase and carbonyl reductase 3 (complete cds)
-
41.1
A
0.999041
63.3
A
0.872394
74.6
A
0.960635
89.8
A
0.872355
330.4
A
0.541191
616.5
P
0.005636
673.4
A
0.098054
355.6
A
0.32083
814
A
0.189648
663.6
A
0.07383
3.55
I
1.00E-06
3.34
NC
0.011266
1.84
NC
0.029473
3.7
I
0.001566
0.9
I
0.000955
9
90
2
40
3
60
0
0
CBR3
carbonyl reductase 3
215_g_at
317.86
1291.96
1
0
1
0.073465
0.024078
0.302001
0.049922
4.11
Up
0.003329008
0.01247248
HUMHOX7 Homo sapiens (region 7) homeobox protein (HOX7) mRNA, complete cds
MSX1
135.9
A
0.918663
540.1
A
0.621816
137.5
A
0.939581
177.5
A
0.781017
598.3
A
0.250724
1993.6
P
0.011447
992.2
A
0.089405
896
A
0.302547
1508.2
A
0.150527
1069.8
A
0.35869
3.17
I
0
1.36
I
0.00224
3.12
MI
0.002825
2.97
NC
0.018117
0.74
NC
0.221643
9
90
2
40
2
40
0
0
MSX1
msh homeo box homolog 1 (Drosophila)
41036_at
626.3
1550.68
0
0
0
0.144092
0.045219
0.364668
0.064909
2.53
Up
0.023619723
0.05564785
dJ1170K4.1 (novel protein similar to KIAA0176 and mouse, worm and fly proteins)
-
398.6
A
0.378184
352.6
A
0.397994
983.8
A
0.35869
1197.7
A
0.32083
198.8
A
0.67917
1665.4
A
0.07383
2173.3
M
0.05447
1701.5
A
0.081337
572
A
0.458816
1641.2
A
0.107301
1.59
I
4.00E-05
2.13
I
6.80E-05
0.63
NC
0.051889
-1.42
NC
0.772757
2.61
I
0.000177
9
90
2
40
3
60
0
0
FLJ12242
hypothetical protein FLJ12242
35792_at
56.9
1119.98
1
0
1
0.013016
0.002203
0.259227
0.035134
19.92
Up
0.000113317
0.001135951
Human lysophospholipase homolog (HU-K5) mRNA, complete cds
MGLL
95.3
A
0.918698
44.2
A
0.968664
54.4
A
0.986921
53.4
A
0.945559
37.2
A
0.951033
1004.7
A
0.098054
937.4
A
0.117121
671.4
A
0.339558
1358.5
A
0.089369
1627.9
P
0.021849
3.86
I
0.000467
4.55
I
0.000868
4.05
NC
0.027053
4.97
I
1.10E-05
5.22
I
0.00057
9
90
1
20
4
80
0
0
MGLL
monoglyceride lipase
40968_at
99.62
734.98
1
0
1
0.022864
0.002021
0.172215
0.041476
7.53
Up
0.007016676
0.02150719
Homo sapiens mRNA for STAT induced STAT inhibitor-3, complete cds
SOCS3
109
A
0.910595
77.8
A
0.960635
108.8
A
0.960635
123.1
A
0.910595
79.4
A
0.951005
565.5
A
0.284747
1282.9
M
0.048995
492.5
A
0.234557
350.7
A
0.715253
983.3
A
0.089405
3.02
I
0.000693
4.17
I
4.00E-05
2.33
I
0.001226
1.73
NC
0.170131
3.86
I
0.000357
9
90
1
20
4
80
0
0
SOCS3
suppressor of cytokine signaling 3
37411_at
1056.72
422.48
0
1
1
0.24325
0.023768
0.097866
0.040176
2.49
Down
0.014347922
0.03723541
Human mRNA for KIAA0050 gene, complete cds
CENTB1
956
A
0.060419
1073.6
A
0.107263
983.6
A
0.339558
1418.6
P
0.039365
851.8
A
0.175989
514.8
A
0.438361
316.3
A
0.561639
110.6
A
0.926204
1071
A
0.458816
99.7
A
0.837105
-1.43
D
0.999159
-1.82
D
0.99943
-2.75
NC
0.996371
-0.69
D
0.999997
-2.54
D
0.99978
9
90
1
20
0
0
4
80
CENTB1
centaurin, beta 1
34376_at
67.48
1022.08
1
0
1
0.015523
0.002357
0.235816
0.043808
15.19
Up
0.001025139
0.005307364
Homo sapiens PKIG mRNA for protein kinase inhibitor gamma, complete cds
PKIG
41.8
A
0.978134
59.9
A
0.980696
57.1
A
0.985077
76.8
A
0.978151
101.8
A
0.795978
831.8
A
0.060419
729.8
A
0.07383
615.1
A
0.234557
1180.3
P
0.035163
1753.4
A
0.07383
4.23
I
0.000107
3.95
I
8.00E-06
3.66
I
0.001566
4.35
I
6.80E-05
3.36
I
0.000841
9
90
0
0
5
100
0
0
PKIG
protein kinase (cAMP-dependent, catalytic) inhibitor gamma
36811_at
82.48
898.3
1
0
1
0.018856
0.013667
0.209024
0.026607
11.09
Up
0.000218829
0.00181913
Human lysyl oxidase-like protein gene
-
22.2
A
0.94553
15.6
A
0.968664
16.5
A
0.999438
36.3
A
0.980713
321.8
A
0.732537
1101.1
P
0.011447
562.5
A
0.107301
842.6
A
0.066865
1216.1
A
0.107301
769.2
A
0.081337
5.05
I
9.00E-06
4.2
I
0.000382
5.12
I
4.00E-05
4.66
I
0.000896
1.22
I
7.90E-05
9
90
0
0
5
100
0
0
LOXL1
lysyl oxidase-like 1
1748_s_at
295.68
3083.2
1
0
1
0.067654
0.034808
0.721167
0.062223
10.66
Up
1.62E-05
0.000329755
CH19HHR23 Homo sapiens DNA from chromosome 19p13.2 cosmids R31240, R30272 and R28549 containing the EKLF, GCDH, CRTC, and RAD23A genes, genomic sequence
-
138.8
A
0.892699
116.2
A
0.951005
162.7
A
0.939581
154.6
A
0.918663
906.1
A
0.438361
3586.2
A
0.060419
2722.5
A
0.060419
3667.1
P
0.024711
2454.3
A
0.204022
2985.9
A
0.127645
5.09
I
1.20E-05
4.72
I
1.40E-05
4.65
I
0
4.13
I
0.000191
1.34
MI
0.002517
9
90
0
0
4
80
0
0
KLF1
Kruppel-like factor 1 (erythroid)
41743_i_at
47.5
598.96
1
0
1
0.010924
0.003535
0.140004
0.020895
12.82
Up
0.000292372
0.002182122
Homo sapiens FIP2 alternatively translated mRNA, complete cds
OPTN
23.8
A
0.889617
46
A
0.860646
24.8
A
0.980408
35.6
A
0.924084
107.3
A
0.844552
456.8
A
0.155448
713.4
P
0.016523
769.2
P
0.019622
323
A
0.324822
732.4
A
0.066419
4.39
NC
0.013131
4.04
I
0.000218
4.41
I
0.000318
3.64
NC
0.015931
2.6
I
0.001544
8
80
2
40
3
60
0
0
OPTN
optineurin
596_s_at
788.4
180.48
0
1
1
0.181583
0.041776
0.042547
0.011161
4.27
Down
0.01232428
0.033075
HUMGCSFR3 Human granulocyte colony-stimulating factor receptor (CSF3R) mRNA, complete cds
HSU43885 Human Grb2-associated binder-1 mRNA, complete cds
GAB1
386.6
A
0.089405
202.3
A
0.378165
211.7
A
0.660468
59.9
A
0.795978
36
A
0.824051
414.5
A
0.218983
382.9
A
0.204022
430.2
M
0.043968
302.8
A
0.47938
439.1
A
0.073796
-0.21
NC
0.5
0.42
NC
0.28107
1.05
NC
0.205324
1.58
NC
0.300289
3.02
NC
0.09528
9
90
5
100
0
0
0
0
GAB1
GRB2-associated binding protein 1
31418_at
498.68
226.12
1
1
2
0.114424
0.021274
0.053283
0.012162
2.15
Down
0.037226902
0.07856451
Human DNA sequence from PAC 388M5 on chromosome 22. Contains a 60S Ribosomal protein L1 like pseudogene, a chromosomal protein HMG-17 like gene, a Sulfotransferase (Sulfokinase) like gene, a putative GS2 like gene, a predicted CpG island, ESTs and ST
-
427.7
A
0.11716
376.7
A
0.189687
837.2
P
0.017001
551.5
P
0.021866
300.3
A
0.189687
189.2
A
0.07383
368.8
P
0.005636
298.6
A
0.284747
81.2
A
0.35869
192.8
A
0.302547
0.04
NC
0.5
0.11
NC
0.5
-1.3
NC
0.966561
-1.72
NC
0.829869
-0.53
NC
0.411228
7
70
5
100
0
0
0
0
HMGN2
high-mobility group nucleosomal binding domain 2
36435_at
399.32
191.58
1
1
2
0.091655
0.008101
0.044988
0.006344
2.04
Down
0.00191025
0.008400991
Homo sapiens protein phosphatase 2C alpha 2 mRNA, complete cds
PPM1A
460
P
0.008689
315.6
A
0.073695
323.7
A
0.267428
496.4
P
0.005636
400.9
A
0.073796
180.5
A
0.284715
264.1
A
0.117121
226
A
0.234519
120.2
A
0.781017
167.1
A
0.320802
-1.14
NC
0.980188
-0.16
NC
0.350735
-0.58
NC
0.433177
-1.75
NC
0.996649
-0.94
NC
0.799953
8
80
5
100
0
0
0
0
PPM1A
protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
41815_at
54.64
451.94
1
1
2
0.01241
0.005461
0.104686
0.019278
8.44
Up
0.001743058
0.007851439
Homo sapiens mRNA; cDNA DKFZp434G173 (from clone DKFZp434G173)
SYNE2
94
A
0.795978
16.9
A
0.960635
130.5
A
0.872394
17.2
A
0.983014
14.6
A
0.999044
222.9
A
0.581931
554.5
M
0.048939
324.9
A
0.302547
438.3
A
0.397994
719.1
P
0.035163
0.63
NC
0.306825
4.74
NC
0.006442
1.36
NC
0.127011
4.09
NC
0.007968
4.67
I
6.00E-06
8
80
4
80
1
20
0
0
SYNE2
spectrin repeat containing, nuclear envelope 2
34940_at
28.46
218.22
1
1
2
0.006557
0.000685
0.050981
0.016175
7.78
Up
0.025289464
0.0585696
Homo sapiens mRNA; cDNA DKFZp564O0862 (from clone DKFZp564O0862)