|
| Status |
Public on Feb 24, 2007 |
| Title |
Sensitive Replicate 4 |
| Sample type |
RNA |
| |
|
| Source name |
cell line
|
| Organism |
Homo sapiens |
| Characteristics |
KBM-7/B5
|
| Treatment protocol |
grown in tissue culture medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen column extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 7.5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U95A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the HP GeneArray Scanner .
|
| Description |
See manuscript (Leukemia Research, 2007).
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 22, 2007 |
| Last update date |
Mar 16, 2009 |
| Contact name |
Reinhold Munker |
| E-mail(s) |
rmunke@lsuhsc.edu
|
| Phone |
318-675-8770
|
| Fax |
318-675-4969
|
| Organization name |
LSU Health Sciences Center
|
| Department |
Medicine
|
| Lab |
Division of Hematology/ Oncology
|
| Street address |
1501 Kings Highway
|
| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71130 |
| Country |
USA |
| |
|
| Platform ID |
GPL8300 |
| Series (1) |
| GSE7114 |
Comparative analysis of a CML cell line resistant to cyclophosphamide using oligonucleotide arrays and response to TKI |
|
