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| Status |
Public on Oct 11, 2016 |
| Title |
Gene expression analysis of mESCs following short-term inhibition with PD032, CHIRON, EPZ6438 and GSK-J4 both individually and in combination. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment was designed to determine the extent of gene misregulation in mouse ESCs cultured for 24hrs in different base medias and treated with the indicated inhibitors.
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| Overall design |
Polyadenylated mRNA from mESCs cultured in the indicated culture conditions was hybridised as three independed biological replicates to Agilent SurePrint G3 Mouse GE 8x60K Microarrays (GPL10787). Individual replicates for each of the specified conditions were performed in HV H2b-mCh, HV9.3 and HFHC12.3 cell lines.
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| |
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| Contributor(s) |
Illingworth RS, Hölzenspies JJ, Roske FV, Brickman JM, Bickmore WA |
| Citation(s) |
27723457 |
| |
| Submission date |
Jan 06, 2016 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Rob S Illingworth |
| E-mail(s) |
robert.illingworth@ed.ac.uk
|
| Phone |
01316519640
|
| Organization name |
The University of Edinburgh
|
| Department |
Centre for regenerative Medicine
|
| Lab |
Illingworth
|
| Street address |
Centre for Regenerative Medicine
|
| City |
Edinburgh |
| State/province |
Midlothian |
| ZIP/Postal code |
EH16 4UU |
| Country |
United Kingdom |
| |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE65289 |
Polycomb Underlies Transcriptional Heterogeneity in Lineage Priming of Embryonic Stem Cells |
|
| Relations |
| BioProject |
PRJNA308097 |
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